# SpCas9 Protein; Exemption From the Requirement of a Tolerance
> **Rule** · Final rule. · Published 2025-12-16 · Effective 2025-12-16 · 90 FR 58167
## Document
- **Document number:** 2025-22927
- **Category:** pesticide
- **Federal Register citation:** 90 FR 58167
- **CFR reference:** 40 CFR 174
- **Publication date:** 2025-12-16
- **Effective date:** 2025-12-16
- **EPA docket:** EPA-HQ-OPP-2025-0212
## Abstract

This regulation establishes an exemption from the requirement of a tolerance for residues of the SpCas9 protein in or on the food and feed commodities of citrus when used as a plant-incorporated protectant (PIP) in citrus. Soil Culture Solutions LLC (d/b/a Soilcea) submitted a petition to EPA under the Federal Food, Drug, and Cosmetic Act (FFDCA), requesting an exemption from the requirement of a tolerance. This regulation eliminates the need to establish a maximum permissible level for residues of SpCas9 protein.

## Source
- [Federal Register document](https://www.federalregister.gov/documents/2025/12/16/2025-22927/spcas9-protein-exemption-from-the-requirement-of-a-tolerance)
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