# LungMap Phase II - Building a multidimensional map of developing human lung

> **NIH NIH U01** · CINCINNATI CHILDRENS HOSP MED CTR · 2020 · $897,310

## Abstract

ABSTRACT
Building a Multidimensional Map of Developing Human Lung – Jeffrey A. Whitsett, M.D. PI
Our LungMAP2 project seeks to work in a collaborative consortium to develop operational pipelines to
study human lung development from the saccular, alveolar stages of morphogenesis at single cell level
of resolution. The project will integrate single cell transcriptomic, chromatin accessibility, 2D and 3D
high-resolution confocal microscopy, immunofluorescence, multiprobe RNA in situ hybridization studies
to create a detailed molecular map of the cells, cell-cell and cell scaffold interactions in their precise
anatomic positions in the developing human lung. Our LungMAP2 Center will work in close concert with
the Data Coordinating Center (DCC), other LungMAP2 Centers and the Human Tissue Procurement
Core (HTPC) to obtain, annotate, preserve and store human tissues using optimal conditions for
imaging and omics analyses. Our LungMAP2 project will produce a detailed structural expression atlas
of multiple regions, niches in the human lung. We will develop and validate new technologies for tissue
clearing, 3D reconstructions of high-resolution images, multi-probe RNA and IF analysis. We will
develop new tissue digestion single cell and single nuclear RNA analyses for precise regional niches
of the normal developing human lung, tissue from and in infants with rare congenital lung disorders.
We will work in a Consortium to develop useful bioinformatic programs, modeling cell-cell, cell matrix
interactions to define the signaling and transcriptional programs building and maintaining organ
structure and function. Working with the DCC and other LungMAP2 Centers, our bioinformatics
pipelines will enable data visualization, interrogation in user friendly web portals for sharing with
members of the LungMAP2 Consortium, the DCC and for the research community in general.

## Key facts

- **NIH application ID:** 10000199
- **Project number:** 5U01HL148856-02
- **Recipient organization:** CINCINNATI CHILDRENS HOSP MED CTR
- **Principal Investigator:** Jeffrey A Whitsett
- **Activity code:** U01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $897,310
- **Award type:** 5
- **Project period:** 2019-08-23 → 2024-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10000199

## Citation

> US National Institutes of Health, RePORTER application 10000199, LungMap Phase II - Building a multidimensional map of developing human lung (5U01HL148856-02). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10000199. Licensed CC0.

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