# Core Grant for Vision Research

> **NIH NIH P30** · WAYNE STATE UNIVERSITY · 2020 · $554,428

## Abstract

Project Summary (Overall)
Continuing funds are requested by 17 vision scientists (holding 13 eligible NEI R01 grants) and
their personnel to support three resource/service cores and an administrative core in the newly
merged Department of Ophthalmology, Visual and Anatomical Sciences (former Ophthalmology
and Anatomy/Cell Biology Departments). The Administrative core will support the overall
management of the Center Core. The research cores will enable and enhance vision research
at Wayne State University and for one member, at nearby (15 minutes) Oakland University and
another at Henry Ford Hospital (5 minutes away). Facilities requested are:
lmaging/Histopathology (I/H), Immunology (I) and Tissue Culture/Molecular (TC/M). The I/H
core in Scott Hall, will provide for confocal laser scanning, light microscopy,
immunofluorescence, OCT and other cell imaging analyses (e.g., cell viability and quantification
of plasma membrane potential), and train in equipment use. There also is assistance/training in
management of digital images, slit lamp photography, and poster and publication production.
The I core, at Kresge Eye Institute, will provide immunological assays and consultation to these
vision researchers. It will initiate and stimulate innovative research projects that address
emerging questions on the immunological basis of many ocular diseases. Among its functions, it
will provide for isolation of peripheral blood cells for staining or viable cryopreservation,
immunophenotyping, phagocytosis assays, ELISA and Western blot evaluation of
cytokines/chemokines and other molecules from tissue lysates and culture supernates, image
analysis for Western blots, data management and storage, and access to the C6 Accuri Flow
cytometer and Cellometer instruments and to the Karmanos Flow facility. The TC/M facility,
located in Scott Hall, will assist and train for preparation of specialized media, isolation,
purification and characterization of ocular cells for primary culture, subculture and propagation
of established cell lines, adherence assays, biofilm evaluation using crystal violet staining,
genotyping of mice, cryopreservation of cells, and HEK cell culture for AAV vector packaging
and purification, DNA cloning, site directed mutagenesis, and DNA transfection. Assistance and
training is also available for techniques in molecular biology including PCR array and real time
RT-PCR, storage of bacteria (including multi-drug resistant isolates), vectors and cDNA
constructs. Each of the cores is staffed by well-trained research assistants and directors are
NEI-R01 supported. The core directors and PI, as a team, prioritize NEl R01 supported studies,
promote collaboration, and assist in gathering preliminary data for new NEI R01 submissions.

## Key facts

- **NIH application ID:** 10000926
- **Project number:** 5P30EY004068-37
- **Recipient organization:** WAYNE STATE UNIVERSITY
- **Principal Investigator:** LINDA D HAZLETT
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $554,428
- **Award type:** 5
- **Project period:** 1997-04-01 → 2024-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10000926

## Citation

> US National Institutes of Health, RePORTER application 10000926, Core Grant for Vision Research (5P30EY004068-37). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10000926. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*