# Tissue Culture/Molecular (TC/M) Core

> **NIH NIH P30** · WAYNE STATE UNIVERSITY · 2020 · $118,440

## Abstract

Project Summary (Tissue Culture/Molecular)
Continuing funds are requested by 17 vision scientists (holding 13 eligible NEI R01 grants) and
their personnel to support three resource/service cores and an administrative core in the newly
merged Department of Ophthalmology, Visual and Anatomical Sciences (former Ophthalmology
and Anatomy/Cell Biology Departments). The Tissue Culture/Molecular (TC/M) core will be
directed by Dr. Jena Steinle, an R01-funded investigator with significant expertise in tissue
culture and molecular biology techniques. Together with the PI, and a research assistant, the
environment and capability to conduct vision research at Wayne State University and affiliated
institutions will be enhanced through prioritization of the work of NEI R01 funded investigators,
their staff and students (graduate and fellows) in numerous aspects of both tissue culture and
molecular biology. The TC/M core will provide vision scientists (priority for NEI R01 funded) with
facilities and technical assistance that will assure quality and cost effectiveness of the work
performed. This core will maintain and propagate ocular and other cell types, as well as provide
investigators with genotyping, reverse transcription-polymerase chain reaction (RT) and
quantitative polymerase chain reaction (q)-PCR, gene silencing- siRNA, shRNA, CRISPR/Cas9,
nested PCR and adherence assays. It will also provide for consultation/assistance on
recombinant (r) AAV virus vector design and construction and assistance to the PI, students or
staff for in vitro functional assessments. Overall, the TC/M core will assist NEI R01-funded
investigators and their staff/students in studies that require the use of tissue culture and
molecular biology techniques; foster collaboration between vision scientists using tissue culture
and molecular biological techniques; and bring new investigators into vision research by
providing assistance in tissue culture and molecular techniques that can expand their research
focus.

## Key facts

- **NIH application ID:** 10000934
- **Project number:** 5P30EY004068-37
- **Recipient organization:** WAYNE STATE UNIVERSITY
- **Principal Investigator:** Jena J Steinle
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $118,440
- **Award type:** 5
- **Project period:** 1997-04-01 → 2024-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10000934

## Citation

> US National Institutes of Health, RePORTER application 10000934, Tissue Culture/Molecular (TC/M) Core (5P30EY004068-37). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10000934. Licensed CC0.

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