# Mechanism of heterochromatin regulation by STAT

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 2020 · $315,167

## Abstract

Mechanism of heterochromatin regulation by STAT
Heterochromatin is a tightly packed form of DNA important for chromosomal compaction and transcriptional
silencing as well as for genome stability, animal longevity, and tumor suppression. How heterochromatin
dynamics, i.e., its establishment, maintenance, and loss, is controlled remains incompletely understood. We
have previously demonstrated a physiological role of uSTAT in heterochromatin maintenance, but the
mechanism remains unclear. The overall goal of this project is to investigate, at the molecular level, the role of
STAT in establishing and maintaining heterochromatin, and to understand how STAT phosphorylation can
serve as a molecular switch converting gene silencing to active transcription. We have previously shown by
immunostaining that a fraction of STAT not phosphorylated at the critical tyrosine around amino acid 700
(termed uSTAT) is localized in the nucleus in association with Heterochromatin Protein 1 (HP1). We have
shown genetically that STAT is essential for heterochromatin maintenance, and that STAT activation (by
phosphorylation on this tyrosine) is associated with heterochromatin disruption. We have further shown that
human uSTAT5A is capable of promoting heterochromatin formation and suppressing tumor growth. In new
preliminary studies using chromatin immunoprecipitation followed by deep sequencing (ChIP-seq), we have
found that the majority of chromatin-bound Drosophila STAT is localized in heterochromatin, and that loss of
STAT leads to a global decrease in heterochromatin, which is marked by trimethylated histone 3 at lys9
(H3K9me3). We have further found that, when forced to bind to euchromatin, uSTAT can repress nearby
genes in an HP1-dependent manner. These results suggest that uSTAT may play an important role not only in
maintaining but also in initiating heterochromatin formation. HP1 and H3K9me3 are hallmarks of
heterochromatin, with HP1 being the central component. It has been shown that recruiting HP1 to euchromatin
is sufficient for heterochromatin formation. However, HP1 does not bind DNA directly and has only weak
affinity for H3K9me3, and thus may require DNA-binding protein factors for its initial recruitment to
heterochromatic loci, or for strengthening its binding to H3K9me3. We hypothesize that uSTAT plays roles in
both establishment and maintenance of heterochromatin due to the molecular confirmation of uSTAT dimers.
We will use a combination of genomic, genetic, and biochemical tools to investigate the initial events leading to
heterochromatin establishment, the respective roles of STAT and HP1 in heterochromatin maintenance, the
requirement for cis-element in uSTAT binding and HP1 recruitment, and the properties of uSTAT and pSTAT
dimers in DNA and HP1 binding. Understanding the molecular mechanism of noncanonical STAT function in
heterochromatin regulation should shed light on not only the basic cell biological process of heterochromatin
dynamics but al...

## Key facts

- **NIH application ID:** 10003343
- **Project number:** 5R01GM131044-03
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN DIEGO
- **Principal Investigator:** WILLIS X LI
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $315,167
- **Award type:** 5
- **Project period:** 2018-09-20 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10003343

## Citation

> US National Institutes of Health, RePORTER application 10003343, Mechanism of heterochromatin regulation by STAT (5R01GM131044-03). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10003343. Licensed CC0.

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