# Analytic Core

> **NIH NIH P20** · MEDICAL UNIVERSITY OF SOUTH CAROLINA · 2020 · $153,177

## Abstract

CORE E: ANALYTICAL REDOX BIOCHEMISTRY
Director: Danyelle Townsend, PhD
ABSTRACT
Understanding the complexities of redox mediated signaling events requires a multidisciplinary
approach. The SC COBRE in Oxidants, Redox Balance and Stress Signalling has assembled a
cohort of promising junior faculty with expertise in relevant biomedical model systems. Analytical
biochemistry specific to the detection and quantification of redox sensitive molecules and coordinate
protein changes that drive homeostasis is a unique niche. As such, development of the Analytical
Redox Biology Core (ARBC) is important. The primary objective of the Core is to provide
comprehensive analytical redox biochemistry methods and mentoring support for the COBRE junior
faculty with the goal to advance their research endeavors, publications and fundability. The specific
aims of the ARBC are: 1) Provide ROS/RNS identification and quantification using state-of-the-art
techniques; 2) Perform quantitative analysis of ROS/RNS (redox molecules and metabolites),
including those associated with calcium mobilization and changes in energy metabolism; 3) Provide
expertise and technology for in depth biochemical analysis of thiol-centered enzyme activities and
define protein:protein interactions. Since oxidative (nitrosative) stress often is associated with a
conditional increase in antioxidant protection, the Core has established methods to detect and
measure various antioxidant enzyme activities as a function of oxidant stress/antioxidant protection
equilibrium. Comprehensive analysis of redox status also includes measurement of intracellular GSH,
GSSG, protein surface and “buried” thiols utilizing both endpoint and/ or real-time kinetic
measurements with millisecond resolution. In complex studies of redox signaling, certain
protein:protein interactions appear to be redox dependent and attributed to post-translational
modifications, including S-nitrosylation and S-glutathionylation. The ARBC has developed fluorescent
labeling and FRET analysis to evaluate redox dependent protein:protein interactions with subsequent
in silico molecular modeling using ZDOCK, GOLD Suite (v 5.2) software. Collectively, these
technologies will provide a multidisciplinary approach to advance the understanding of redox
mediated signaling events specific to the model systems presented by the junior faculty in their
research.

## Key facts

- **NIH application ID:** 10005394
- **Project number:** 5P20GM103542-10
- **Recipient organization:** MEDICAL UNIVERSITY OF SOUTH CAROLINA
- **Principal Investigator:** Danyelle M. Townsend
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $153,177
- **Award type:** 5
- **Project period:** 2011-09-01 → 2022-01-15

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10005394

## Citation

> US National Institutes of Health, RePORTER application 10005394, Analytic Core (5P20GM103542-10). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10005394. Licensed CC0.

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