# Proteomics Core

> **NIH NIH P20** · MEDICAL UNIVERSITY OF SOUTH CAROLINA · 2020 · $153,176

## Abstract

ABSTRACT
The objective of Proteomics Core B is to provide state of the art mass spectrometry-based proteomic
capabilities, dedicated analytical support, and mentorship to the investigators funded through the SC COBRE
in Oxidants, Redox Balance, and Stress Signaling (Redox COBRE). Mass spectrometry is a powerful
technology enabling unbiased discovery of the effects of drugs/ligands, genetic alterations, and disease on
changes in protein expression, protein interactions, and post-translational modifications. Post-translational
modifications encode the nutrient and stress status of the cell and mediate acute responses to ligand
stimulation and changes in redox status by influencing protein function, half-life, and localization. The MUSC
Mass Spectrometry Facility and Proteomics Center have an extensive array of instrumentation for protein
characterization and expertise in applying proteomic technologies to biomedical research endeavors. During
the last funding period acquisition of an Orbitrap Elite mass spectrometer, purchased through a shared
instrumentation grant, enabled us for the first time to apply quantitative proteomic approaches to the detection
of complex post-translational modifications. In ongoing collaborative projects with COBRE investigators and
participants in the Redox Center, Proteomics Core B is utilizing the Orbitrap Elite to examine the global effects
of altered redox status on protein expression, phosphorylation, acetylation, and thiol modifications and to
characterize the sites of phosphorylation, S-glutathionylation, tyrosine nitration, and protein crosslinking on
redox-sensitive target proteins. The specific aims of Proteomics Core B are: 1) to assist COBRE investigators
with experimental design including sample preparation strategies and choice of specific proteomic approach, 2)
to customize data acquisition and analysis to address the goals of the individual projects, and 3) to provide
mentorship and training in the applications of proteomic technologies for future projects. Access to these
capabilities and expertise to push the available technology forward is critical to the proposed research projects.
The experimental capabilities and infrastructure established with COBRE investigators are made available to
investigators at MUSC and other institutions in South Carolina that utilize the MUSC Mass Spectrometry
Facility. Our goals are to provide COBRE investigators with access to instrumentation and expertise to
advance their research and contribute to the success of our promising junior faculty.

## Key facts

- **NIH application ID:** 10005398
- **Project number:** 5P20GM103542-10
- **Recipient organization:** MEDICAL UNIVERSITY OF SOUTH CAROLINA
- **Principal Investigator:** Lauren Elizabeth Ball
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $153,176
- **Award type:** 5
- **Project period:** 2011-09-01 → 2022-01-15

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10005398

## Citation

> US National Institutes of Health, RePORTER application 10005398, Proteomics Core (5P20GM103542-10). Retrieved via AI Analytics 2026-05-28 from https://api.ai-analytics.org/grant/nih/10005398. Licensed CC0.

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