# Mass Spectometry and Proteomics

> **NIH NIH P30** · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · 2020 · $227,159

## Abstract

SUMMARY for the Mass Spectrometry and Proteomics Core
Developments in mass spectrometry, primarily electrospray ionization (ESI) liquid chromatography-mass
spectrometry (LCMS) with tandem mass spectrometry (MS/MS) has powered the remarkable growth in the
fields of both proteomics and metabolomics. Because proteomics and metabolomics measure the dynamic
state of cells and animals, they are powerful techniques for studying and discovering mechanisms for a range
of pathophysiologic states from cancer to immunology. The primary goal of the Mass Spectrometry and
Proteomics Core is to provide VCIID-COBRE investigators with support for experiments based on mass
spectrometry-based analyses in the areas of proteomics and metabolomics. We provide support for
experimental design, sample preparation, data acquisition, data analysis, and interpretation of data obtained.
Services include proteomic analyses with a focus on quantification of proteins and posttranslational
modifications, small molecule quantification, metabolite pattern identification, and measurement of rates and
kinetics using stable isotopes of both metabolites and proteins. These services are based upon over 40 years
of experience in these areas leading core facilities by the Core Director, Dr. Matthews. The Mass
Spectrometry and Proteomics Core has been pivotal to the success of VCIID-COBRE faculty by contributing
critical information for publications and grants during Phases I and II of the VCIID-COBRE program. With
instrumentation expansion at the end of Phase II with the addition of a high resolution Waters Xevo G2-XS
ESI-LCMS/MS quadrupole-time of flight instrument and Waters nanoAcquity UPLC, the Core has been able to
expand its capabilities and services going into Phase III. The aims of the Core for Phase III are (1) to provide
state-of-the-art mass spectrometry, cutting-edge techniques, and experienced personnel to enhance the
research of the VCIID-COBRE. In addition to the standard protein analyses already conducted, VCIID-COBRE
faculty has identified new techniques that are required for their future investigations. These include
development of a broad-based platform for metabolomics measurements and measurement of metabolite
kinetics and pathways using stable isotopically labeled tracers and broadening our capabilities to provide
identification and quantification using stable isotope labels of difficult to define posttranslational modifications
and rates of protein turnover. (2) To provide an infrastructure of consultative services to VCIID-COBRE faculty
in the design, conduct, and interpretation of experiments to facilitate their research and (3) To develop a long-
term plan for sustainability through expansion of user base with a fee-for-service structure, strong institutional
support, and adaptation to changes in research needs.

## Key facts

- **NIH application ID:** 10006839
- **Project number:** 5P30GM118228-05
- **Recipient organization:** UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
- **Principal Investigator:** Dwight E Matthews
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $227,159
- **Award type:** 5
- **Project period:** 2016-08-05 → 2023-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10006839

## Citation

> US National Institutes of Health, RePORTER application 10006839, Mass Spectometry and Proteomics (5P30GM118228-05). Retrieved via AI Analytics 2026-08-03 from https://api.ai-analytics.org/grant/nih/10006839. Licensed CC0.

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