# CSE regulation of vascular remodeling

> **NIH NIH R01** · LOUISIANA STATE UNIV HSC SHREVEPORT · 2020 · $427,993

## Abstract

Project Summary
Hydrogen sulfide synthesis and metabolism is an important participant in cardiovascular health and function.
Specifically, our laboratory has shown that cystathionine g-lyase (CSE) expression and function play a critical
role in ischemic vascular remodeling responses of arteriogenesis and angiogenesis. Moreover, our group has
revealed important chemical biology and pathophysiological relationships between sulfide and nitric oxide
metabolites in clinical vascular disease conditions, which may be important for cooperative regulation of
ischemic vascular remodeling. However, numerous molecular and cellular mechanisms remain completely
unknown in these responses including: the role of specific cell populations in producing discrete sulfide species
during ischemic vascular remodeling, how different sulfide metabolites modulate nitric oxide (NO)
bioavailability through various biochemical pathways, and mechanisms regulating rapid increases in CSE
activity dependent sulfide metabolite bioavailability during hypoxia. This application will address these
important unknown areas using novel tissue specific CSE mutant mouse models, cutting edge analytical
chemistry measurement methods of sulfide and NO species, cellular and molecular methods to discover
posttranslational regulation of CSE protein activity in response to hypoxia, and clinical tissue specimens to
better understand persulfide and polysulfide during vascular remodeling and disease. Using the models and
tools above, this proposal will examine the hypothesis that endothelial cell and monocyte CSE dependent
polysulfide formation differentially regulates ischemic vascular remodeling and NO bioavailability. Three
specific aims will be pursued to test the hypothesis including: 1) determine the mechanisms of endothelial CSE
regulation of ischemic vascular remodeling and how it controls vascular cell NO bioavailability, 2) determine
the mechanisms of monocyte CSE regulation of arteriogenesis, and 3) determine mechanisms of CSE activity
and expression in experimental models and clinical specimens. Completion of this project will provide crucial
new basic insight that is not currently available regarding mechanisms of CSE regulation and polysulfide
effects on cell biology during ischemic vascular remodeling.

## Key facts

- **NIH application ID:** 10007007
- **Project number:** 1R01HL149264-01A1
- **Recipient organization:** LOUISIANA STATE UNIV HSC SHREVEPORT
- **Principal Investigator:** Christopher G Kevil
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $427,993
- **Award type:** 1
- **Project period:** 2020-07-01 → 2024-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10007007

## Citation

> US National Institutes of Health, RePORTER application 10007007, CSE regulation of vascular remodeling (1R01HL149264-01A1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10007007. Licensed CC0.

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