# Role of an Integrator-EGR axis in the regulation of myeloid enhancers (Admin Supp)

> **NIH NIH R01** · WISTAR INSTITUTE · 2020 · $101,007

## Abstract

Project Summary
Mono/macrophagic commitment of myeloid cells comprises two distinct and largely independent
developmental steps. First, hematopoietic stem cells asymmetrically divide to generate a succession of
lineage-restricted progenitors that culminate in differentiation of peripheral, post-mitotic monocytes. Second,
blood-circulating monocytes are short-lived cells that can undergo differentiation into macrophages, which are
specialized phagocytic cells capable of infiltrating most tissues. While monocytes can mediate inflammation
and cell toxicity, to some extent, macrophages boast the widest set of defense mechanisms against pathogens
and can elicit a robust inflammatory response.
In this proposal, we analyze the molecular determinants of monocytic and macrophagic development by
profiling the zinc-finger EGR1 transcription factor that has been previously implicated in myeloid cell
development. EGR1 is essential for development of monocytes and binds a set of critical enhancers that
regulate monocytic differentiation. In macrophages, however, EGR1 boasts an additional repressor function at
inflammatory enhancer elements. We propose to dissect the scope of action of EGR1 in macrophages and
identify the protein partners that cooperate with EGR1 to regulate the epigenome. Furthermore, we propose to
elucidate the chromosome conformation changes that accompany late-stage development of myeloid cells and
determine how they impact a macrophage’s ability of promoting inflammation.

## Key facts

- **NIH application ID:** 10007301
- **Project number:** 3R01HL141326-02S1
- **Recipient organization:** WISTAR INSTITUTE
- **Principal Investigator:** Alessandro Gardini
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $101,007
- **Award type:** 3
- **Project period:** 2020-03-08 → 2023-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10007301

## Citation

> US National Institutes of Health, RePORTER application 10007301, Role of an Integrator-EGR axis in the regulation of myeloid enhancers (Admin Supp) (3R01HL141326-02S1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10007301. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*