# Method validation for the detection of aflatoxins in fish feed using the ROSA-M Charm reader

> **NIH FDA U18** · UNIVERSITY OF PENNSYLVANIA · 2020 · $45,104

## Abstract

PROJECT SUMMARY/ABSTRACT
The PADLS New Bolton Center Toxicology Laboratory proposes to expand the capacity for detection of
mycotoxins in animal feeds through the analysis of aflatoxins in fish feed using the hand-held Charm
ROSA-M reader. We have previously validated this qualitative rapid lateral-flow method for the
detection of aflatoxins in dog food as outlined in the Guidelines for the Validation of Chemical Methods
for the FDA FVM Program, 2nd Edition.
After completing the validation for aflatoxins in our laboratory, samples will be sent out for validation to
3 other collaborating Vet-LIRN laboratories. Each collaborating Vet-LIRN laboratory will report their
analytical results accompanied by any method modifications or analytical problems. The results will be
statistically evaluated and results from each laboratory will be evaluated for major discrepancies,
displaced means, and any outlying values.
Upon review of the data reported by the collaborating Vet-LIRN laboratories, a standardized method
standard operating procedure (SOP) for the analysis of aflatoxins in fish feed using the Charm ROSA-M
reader will be prepared and submitted to the Vet-LIRN Program Office for review and eventual method
transfer to other Vet-LIRN laboratories.

## Key facts

- **NIH application ID:** 10009330
- **Project number:** 5U18FD006856-02
- **Recipient organization:** UNIVERSITY OF PENNSYLVANIA
- **Principal Investigator:** Lisa Murphy
- **Activity code:** U18 (R01, R21, SBIR, etc.)
- **Funding institute:** FDA
- **Fiscal year:** 2020
- **Award amount:** $45,104
- **Award type:** 5
- **Project period:** 2019-09-05 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10009330

## Citation

> US National Institutes of Health, RePORTER application 10009330, Method validation for the detection of aflatoxins in fish feed using the ROSA-M Charm reader (5U18FD006856-02). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10009330. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*