# The Aquaporin-3/Phospholipase D2 Signaling Pathway in Corneal Wound Healing

> **NIH VA I01** · CHARLIE NORWOOD VA MEDICAL CENTER · 2020 · —

## Abstract

Project Summary/Abstract
 Previous data from our laboratory indicate that the lipid-metabolizing enzyme phospholipase D2 (PLD2)
and the glycerol transporter aquaporin-3 (AQP3) physically and functionally interact in epidermal keratinocytes.
AQP3 transports glycerol into the cell, making it available to the associated PLD2, which converts it to
phosphatidylglycerol (PG). Our results further demonstrate that PG acts as a lipid second messenger to
promote epidermal wound healing. Verkman and colleagues have generated an AQP3 knockout mouse model,
which shows a profound epidermal phenotype including delayed skin wound healing. Interestingly, and of
direct significance to this application, these AQP3 knockout mice also exhibit impaired corneal wound healing.
Inspection of the literature suggests that epidermal keratinocytes and corneal epithelial cells show a number of
similarities and may be regulated by common mechanisms. In exciting preliminary data we have found that
PLD2 and AQP3 also associate in corneal epithelial cells and that the product of this interaction, PG, enhances
scratch wound closure of corneal epithelial cells in culture as well as corneal wound healing in wild-type and
AQP3 knockout mice in vivo. These findings lead us to hypothesize that the PLD2/AQP3/PG signaling module
is important in the cornea. Because these effects of PG are reminiscent of the actions of epidermal growth
factor receptor (EGFR) ligands on corneal epithelium, we specifically hypothesize that PLD2 and AQP3
associate in corneal epithelial cells to produce PG in response to EGFR ligands and that this PG acts as a
signaling molecule to accelerate wound healing in the cornea by increasing epithelial cell migration and/or
proliferation. In the research proposed, we will test this hypothesis and define the role of the PLD2/AQP3
signaling module in corneal cell biology and wound healing. Data in the literature and our own initial results in
epidermal keratinocytes indicate that PG can activate protein kinase CβII (PKCβII). Based on these data, as
well as reports that PKCβ inhibition or silencing can inhibit corneal proliferation and proliferative signaling, we
further hypothesize that the mechanism by which the PLD2/AQP3 model functions is through PG-activated
PKCβII activation. We will test this idea using knockout animals, as well as overexpression, RNA interference,
and pharmacological inhibition approaches to increase and decrease the levels of PLD2, AQP3 and PKCβII;
we will then monitor corneal epithelial cell migration and proliferation, PG levels, PKCβII autophosphorylation
(activation) and corneal wound healing in vivo. If our hypothesis proves correct, the research will lead to a
better understanding of the corneal epithelial wound healing process and may lead to the identification of novel
targets for the development of treatments to promote corneal would healing in patients after trauma, infection
or ophthalmic surgery.

## Key facts

- **NIH application ID:** 10016063
- **Project number:** 1I01BX005055-01
- **Recipient organization:** CHARLIE NORWOOD VA MEDICAL CENTER
- **Principal Investigator:** Wendy B Bollag
- **Activity code:** I01 (R01, R21, SBIR, etc.)
- **Funding institute:** VA
- **Fiscal year:** 2020
- **Award amount:** —
- **Award type:** 1
- **Project period:** 2020-07-01 → 2024-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10016063

## Citation

> US National Institutes of Health, RePORTER application 10016063, The Aquaporin-3/Phospholipase D2 Signaling Pathway in Corneal Wound Healing (1I01BX005055-01). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10016063. Licensed CC0.

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