# Bloomington Drosophila Stock Center at Indiana University

> **NIH NIH P40** · TRUSTEES OF INDIANA UNIVERSITY · 2020 · $40,136

## Abstract

Project Summary
The Bloomington Drosophila Stock Center (BDSC) supports a large, worldwide community of scientists using
the insect Drosophila melanogaster as a model organism for biomedical experimentation. Its primary missions
are to provide a collection of documented living stocks of broad value to current research, to preserve
documented strains with clear future value, and to provide information and support services that promote
maximal exploitation of these materials. The goal of this revision proposal is to continue making a large set of
Drosophila strains available through the BDSC for the controlled expression of any UAS-based genetic
construct in a small subset of cells defined by the intersecting expression patterns of two “split-GAL4” driver
constructs. In the split-GAL4 system, one driver construct expresses the GAL4 DNA-binding domain fused to a
protein-pairing domain and the other construct produces a transcriptional activation domain fused to the
complementary protein pairing domain. In cells where the expression patterns of the two drivers coincide,
dimerization can occur to generate an active GAL4 and UAS transgenes can be expressed. This intersectional
strategy produces more refined and specific expression patterns than those produced by single drivers. This
system has been used to define and manipulate the functions of specific neuron types in investigations of brain
wiring and it has the potential to identify unique subsets of cells in many other tissues and allow them to be
manipulated experimentally. The funding proposed here would make it possible for the BDSC to continue
maintaining, distributing and promoting the use of an extensive set of stocks with split-GAL4 transgenes, which
were generated at considerable effort and cost by investigators at Janelia Research Campus and were
transferred to the BDSC for distribution under an administrative supplement in September 2017. Funding will
make this large collection available to the full biomedical research community and will provide new
opportunities to identify cell populations, explore their functions and use them in developing models of human
disease processes.

## Key facts

- **NIH application ID:** 10018147
- **Project number:** 3P40OD018537-06S1
- **Recipient organization:** TRUSTEES OF INDIANA UNIVERSITY
- **Principal Investigator:** KEVIN R COOK
- **Activity code:** P40 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $40,136
- **Award type:** 3
- **Project period:** 2014-08-01 → 2024-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10018147

## Citation

> US National Institutes of Health, RePORTER application 10018147, Bloomington Drosophila Stock Center at Indiana University (3P40OD018537-06S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10018147. Licensed CC0.

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