# Biochemistry & Molecular Biology  Instrumentation Core

> **NIH NIH P30** · UNIVERSITY OF CALIFORNIA LOS ANGELES · 2020 · $34,989

## Abstract

PROJECT SUMMARY – BMBI CORE
The Biochemistry and Molecular Biology Instrumentation (BMBI) module is an essential core
resource that continues to be heavily used by the majority of our investigators to support the
preparation and analysis of biological samples. The BMBI core module resources consist of
common instrumentations such as autoclaves, various spectrophotometers, centrifuges,
incubators, microplate readers, immunoassay protein analyzers, polymerase chain reaction
(PCR) machines for DNA and RNA analysis. While most of these instruments are basic, their
utilization is extensive and critically important for the progress of research programs in each
laboratory. The majority of our core investigators employ basic biochemistry and molecular
biological approaches to prepare and analyze quantitatively and qualitatively various biological
samples. These include RNA, DNA, and protein samples obtained directly from tissue
specimens or extracted from heterologous cell- and non-cell-based protein expressing systems.
Besides the common instruments, this module has available specialized spectrometers for
detailed protein structural and functional analysis, including fluorescence, UV-visible
absorbance, Fourier transform infra-red, and customized circular dichroism (CD)
spectroscopies. In addition, sophisticated multi-angle and quasi-elastic light scattering
instruments are available. Our core investigators can determine gene expression profiles using
high throughput microfluidic assays, a next generation single cell RNA-sequencing (scRNA-seq)
PCR approach. A recently purchased automated Western immunoblot system allows for
quantification of low abundant proteins via a sensitive immunoassay reaction involving minimal
sample manipulation with data being analyzed digitally. Our newly acquired Molecular Devise
SpectraMax iD3 microplate reader permits quantification at multiplex formats (6 to 384-well) for
absorbance, fluorescence, and luminescence, thus enabling multiple biochemical assays at the
same time.
The BMBI module infrastructure and instrumentation capabilities are quintessential for our
investigators day-to-day scientific productivity by stimulating interactions among our researchers
and facilitating the quick integration of new recruits.

## Key facts

- **NIH application ID:** 10020830
- **Project number:** 2P30EY000331-53
- **Recipient organization:** UNIVERSITY OF CALIFORNIA LOS ANGELES
- **Principal Investigator:** ROXANA A RADU
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $34,989
- **Award type:** 2
- **Project period:** 1997-03-01 → 2025-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10020830

## Citation

> US National Institutes of Health, RePORTER application 10020830, Biochemistry & Molecular Biology  Instrumentation Core (2P30EY000331-53). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10020830. Licensed CC0.

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