# Flow Cytometry Shared Resource

> **NIH NIH P30** · COLUMBIA UNIVERSITY HEALTH SCIENCES · 2020 · $125,440

## Abstract

FLOW CYTOMETRY SHARED RESOURCE: PROJECT SUMMARY
The Flow Cytometry Shared Resource (FCSR) gives access to flow cytometry analysis and cell sorting
instruments and services to Herbert Irving Comprehensive Cancer Center (HICCC) members. Under the
leadership of Remi Creusot, PhD, the FCSR provides: (1) multi-color panel flow data acquisition and analysis
using flow cytometry analyzers, including high throughput screening (HTS), (2) flow cytometry cell sorting (four-
way or six-way or plate) using high speed cell sorters by dedicated operators of FCSR, including single cell index
sorting, and (3) comprehensive consultation, panel design, hands-on training, troubleshooting, and data analysis
services by PhD-level FCSR scientists. This broad and inclusive package of services enables HICCC
investigators to analyze tumor cell populations and their microenvironment, including the tumor-infiltrating
lymphoid and myeloid cells at the single cell level. During the current project period (2014-2019), the FCSR has
been integrated with the flow cytometry core of the Columbia Center for Translational Immunology (CCTI). The
merging of these facilities has secured access to a broader range of state-of-the-art instrumentation and brought
advanced expertise in immunology to the research of HICCC flow cytometry users. HICCC members now have
access to nine instruments, including five analyzers and four sorters. In addition, two of the existing
instruments/analyzers have been upgraded to 18-20 detectors. Moreover, a new Influx sorter has been acquired
to expand the capacity of sorting services. Upgrades in consultation services have further empowered the
research of HICCC members who now benefit from face-to-face consultation on panel design, protocol
optimization, and hands-on training on data acquisition, analysis, and experimental troubleshooting essential to
maximize the impact of new advanced instruments for high dimensional multi-color panels. Based on its
advanced instrumentation, highly qualified staff, broad portfolio of specialized services, easy accessibility, and
cost-effective services, the FCSR is the flow cytometry service provider of choice for HICCC members. The
FCSR will continue to incorporate the latest technologies and to provide high quality cell sorting, analytical and
consultation services to HICCC members to empower their understanding of cancer biology and to enable new
breakthroughs in cancer therapy. Over the current project period, the capabilities of the FCSR have been utilized
by 81 HICCC members, provided key data and insights to support the success of more than 46 HICCC member
peer-reviewed publications, including 23 contributions in journals with impact factor >10, of which 12 were in
journals with an impact factor >20 (Nature, Cell Stem Cell, Cancer Cell, Cancer Discovery), and currently support
research for 21 NIH-funded research grants (11 from NCI).

## Key facts

- **NIH application ID:** 10022769
- **Project number:** 2P30CA013696-45
- **Recipient organization:** COLUMBIA UNIVERSITY HEALTH SCIENCES
- **Principal Investigator:** Remi J Creusot
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $125,440
- **Award type:** 2
- **Project period:** 1997-07-04 → 2025-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10022769

## Citation

> US National Institutes of Health, RePORTER application 10022769, Flow Cytometry Shared Resource (2P30CA013696-45). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10022769. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*