CORE 003 – BIOANALYTICS AND PROTEOMICS SHARED RESOURCE PROJECT SUMMARY/ABSTRACT The mission of the Bioanalytics and Proteomics Shared Resource (BPSR) is to provide cost-effective, state-of- the-art instrumentation and analytical expertise in mass spectrometry to investigators. The BPSR is centrally located on the Vanderbilt campus and is composed of three components that provide the following analytical services: 1) drug and metabolomics analysis, 2) comprehensive proteomics analysis and 3) molecular profiling and tissue imaging. BPSR staff provide consultation on experimental design and sample preparation as well as perform all aspects of mass spectrometry analyses and data analysis. LC-MS/MS, GC-MS and MALDI-TOF instruments are available for small molecule, drug and metabolite analysis. LC-MS/MS instrumentation is available for proteomics analysis, including multiple orbitrap systems. A variety of MALDI-based instruments are provided for molecular profiling and tissue imaging experiments. In addition, inductively coupled plasma mass spectrometry (ICP-MS) capabilities are offered for elemental analysis and tissue imaging. Specific services include identification and quantification of small molecules in biofluids and tissues, identification and quantification of proteins and their modifications by either stable isotope labeling (SILAC and TMT/iTRAQ) or label free methods, crosslinking and hydrogen-deuterium exchange for protein-protein interaction analysis and imaging of small molecules and proteins in tissues using imaging mass spectrometry. The BPSR staff provide education and training in sample preparation, instrument-based experiments and data analysis to VICC investigators and their laboratory personnel. Lastly, the BPSR collaborates with VICC investigators to continue to offer the latest cutting-edge technology and methods in biomolecular mass spectrometry analysis for high- impact cancer discovery.