# Pathway Analysis in Tuberculosis

> **NIH NIH P01** · WEILL MEDICAL COLL OF CORNELL UNIV · 2020 · $2,639,702

## Abstract

Overall, Abstract
Despite the availability of therapy, Mycobacterium tuberculosis (Mtb) is the major infectious killer of adults.
Finding more effective therapeutic strategies will require a better understanding of the fundamental biology of
bacterial growth, metabolism and interaction with the host. This program proposes to understand key pathways
in Mtb biology using advanced genetic and biochemical methods, with a particular focus on complex
pathways important in adaptation to disease-relevant stressors. “Pathways” is defined broadly, including
biochemical, structural and genetic interactions. In many cases, traditional genetic approaches to discover and
understand these pathways are limited, but many of the most critical aspects of Mtb biology are mediated by
such complex genetic mechanisms. This program will leverage new methodology and resources that the
collaborators have developed in the last few years, to discover the constituents of key pathways, attribute
functions to new pathways, and understand the mechanisms which underlie the ability of bacteria to survive and
grow under a variety of conditions, focusing on those encountered during infection. They will also take advantage
of the close collaborations among the investigators who have worked well together for several years. The work
will be organized into four projects and four cores, but considerable crossover is expected, which will enrich each
component.
Project 1. Metabolic adaptations required for growth and virulence of Mtb at acidic pH.
Project 2. Acquisition, synthesis and importance of biotin in Mtb.
Project 3. Defining the RNA processing and degradation pathways of Mtb.
Project 4. Defining cell division pathways in Mtb.
Core A. Metabolomics and lipidomics.
Core B. Biochemistry and enzymology.
Core C. Bioinformatics and data sharing.
Core D. Administration.
All of this work will be facilitated by a highly collaborative group of investigators, almost all of whom have worked
together for many years on a large variety of projects. Both project and core investigators will serve as full-
fledged intellectual contributors, enabling the team to bring a variety of approaches and thinking to bear on
complex biological systems.

## Key facts

- **NIH application ID:** 10024699
- **Project number:** 1P01AI143575-01A1
- **Recipient organization:** WEILL MEDICAL COLL OF CORNELL UNIV
- **Principal Investigator:** SABINE EHRT
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $2,639,702
- **Award type:** 1
- **Project period:** 2020-06-12 → 2025-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10024699

## Citation

> US National Institutes of Health, RePORTER application 10024699, Pathway Analysis in Tuberculosis (1P01AI143575-01A1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10024699. Licensed CC0.

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