# Core B. Biochemistry and Enzymology

> **NIH NIH P01** · WEILL MEDICAL COLL OF CORNELL UNIV · 2020 · $144,539

## Abstract

Core B, Abstract
The overall goal of this program project is to understand key pathways in the biology of Mtb that are important
in the pathogen’s adaptation to disease-relevant stress conditions – including moving from genetic and molecular
characterization to a biochemical understanding of function. Ultimately this requires both purified proteins, and
enzymatic or binding assays. The Sacchettini lab has considerable experience with both protein expression, and
purification as well as with developing biochemical assays to investigate protein function. While they usually
focus on essential enzymes, the methods used can be applied to most biological macromolecules. The
Sacchettini laboratory also serves as an important interface to the TB Structural Genomics Consortium (TBSGC)
and when appropriate they will provide access to structural resources in order to help determine function. They
will produce recombinant protein and develop assays for all projects within the program project, utilizing methods
developed in the lab or published methods. For those cases where there is no first hand or published information,
they will use their general strategy for making soluble and active Mtb proteins, that they have adopted from their
structural genomics pipeline for high throughout cloning, expression and purification.
The Core’s work on defining the biochemical function of the target proteins will rely heavily on interactions with
the metabolomics core (Core A). This will not be an obstacle as these labs currently work closely on several
projects. In addition, the Core will use standard bioinformatic methods to help understand what the amino
sequence suggests about function, in addition to structures of similar proteins. The core will perform two main
activities:
Activity 1: Clone, overexpress and purify recombinant Mtb proteins for functional analysis using in
vitro biochemical characterization and assays.
Activity 2: Develop in vitro biochemical assays for project proteins.

## Key facts

- **NIH application ID:** 10024702
- **Project number:** 1P01AI143575-01A1
- **Recipient organization:** WEILL MEDICAL COLL OF CORNELL UNIV
- **Principal Investigator:** JAMES C SACCHETTINI
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $144,539
- **Award type:** 1
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10024702

## Citation

> US National Institutes of Health, RePORTER application 10024702, Core B. Biochemistry and Enzymology (1P01AI143575-01A1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10024702. Licensed CC0.

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