PROJECT SUMMARY (MAJEWSKI) Mutations in histone and modifier genes in cancers have dramatic downstream effects on the landscape of the epigenome – chromatin state and DNA methylation – with eventual secondary outcomes manifested in gene expression and cellular phenotypes. Modern functional genomics approaches provide invaluable tools to study the effects of this epigenome-mediated cascade. In this Core activity, we will establish and provide a set of genomics tools and computation methods to elucidate the mechanisms through which histone variants impact the epigenome, transcriptome, and the cell identity during neoplastic transformation.