# Characterization of the function and regulation of long noncoding RNA Interferon gamma- anti-sense 1

> **NIH NIH FI2** · NATIONAL INST ARTH/MUSCLOSKEL/SKIN DIS · 2020 · —

## Abstract

Project Summary/Abstract
The idea that long noncoding RNAs (lncRNA) play an important role in regulating gene
expression is a quickly growing concept. The lncRNA IFNG-AS1 is found in a multi-cytokine
locus which includes IFNG and IL22. IFNG-AS1 has been shown to play an important role for
positively regulating the expression of IFNγ expression in both human and mice. Current
research concerning the role for Ifng-as1 suggests its role as a positive regulator of Ifnγ, but
preliminary data outlined in this grant shows examples of discordant expression of Ifng-as1 and
Ifng. This finding suggests an alternative regulation of this lncRNA. The goal of this proposal is
to understand the function and regulation of Ifng-as1 under different cellular settings. In Aim 1
we have proposed to define the relationship between Ifng-as1 and the genome under different
cellular settings and different cellular states using HiChIRP (i.e. CD4+ T cells and plasma cells).
We will also determine what factors are differentially bound to Ifng-as1 through ATAC-seq and
ChIP-seq. We will additionally describe a metabolic role for the regulation of Ifng-as1 in
particular glycolysis and glutaminolysis through the use of metabolic inhibitors and nutrient
supplementation. In Aim 2 we will define proteins bound to Ifng-as1 through RAP-MS and
defined the function of Ifng-as1 by deleting Ifng-as1 and its regulatory elements by using
CRISPR/Cas9 technology. Finally, we will seek to determine a role for Ifng-as1 in an in vivo
setting for ILC3 differentiation and memory development. The findings from this proposed study
will provide novel mechanistic information concerning the regulation of Ifng-as1 and Ifnγ
providing novel targets for therapeutic intervention.

## Key facts

- **NIH application ID:** 10026713
- **Project number:** 1FI2GM137945-01
- **Recipient organization:** NATIONAL INST ARTH/MUSCLOSKEL/SKIN DIS
- **Principal Investigator:** Danielle Alexandria Chisolm
- **Activity code:** FI2 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** —
- **Award type:** 1
- **Project period:** 2020-09-01 → 2023-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10026713

## Citation

> US National Institutes of Health, RePORTER application 10026713, Characterization of the function and regulation of long noncoding RNA Interferon gamma- anti-sense 1 (1FI2GM137945-01). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10026713. Licensed CC0.

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