PROJECT SUMMARY Consumers are increasingly interested in knowing more about their own health. One of the most familiar direct to-consumer diagnostic tests has been the home pregnancy test. We report a practical approach towards developing a new point-of-care diagnostic test for another reproductive health need—the assessment of male fertility. Traditionally, men are less likely to seek medical attention for fertility concerns than their female partners (although millennial males are more interested in fertility testing). A delay in diagnosis of male factor infertility may prolong the infertility treatment process for the couple and result in unnecessary interventions for the female partner. Manual microscopic testing and computer-assisted semen analysis (CASA) technology are expensive and require significant technical skill to use. The idea of an at-home, affordable testing platform to screen for an abnormal semen analysis would obviate the need for a clinic visit and provide preliminary data to guide referrals. Our prototype device has been extensively tested with clinical semen samples. The preliminary results presented are robust and unbiased and patient results are based on blind evaluation of the technology by comparing our microchip results with standard methods. In this Fast Track SBIR project we will further optimize the design parameters and develop a best in class, cellphone-based semen analysis device that could be marketed to a) for home based testing and b) as an inexpensive alternative to CASA in IVF clinics.