# Molecular and Cellular Analytical Core

> **NIH NIH P30** · UNIVERSITY OF COLORADO DENVER · 2020 · $233,250

## Abstract

PROJECT SUMMARY – MOLECULAR AND CELLULAR ANALYTICAL CORE
The overall theme of the Molecular and Cellular Analytical (MCA) core is to support investigation of human and
non-human metabolism from a cellular/molecular perspective. The overall expertise of the PIs provide the
nucleus of expertise around which this core lab has been formed, including in obesity, type 2 diabetes,
lipidomics, and isotope tracer studies (Bergman), RNAseq analysis and interpretation (Shankar and Rudolph),
and mitochondrial function (Jackman). The lipidomic and mass spectrometry core provides quantitative
lipidomic analysis on plasma, cells, and tissues, as well as help with data interpretation, in addition to
determination of isotope enrichment and concentration of metabolites in blood and breath used in isotope
tracer studies. The RNAseq bioinformatic core provides nucleic acid extraction, advanced computational
processing, and pathway, network, disease, and regulator discovery tools for RNAseq and related sequencing
experiments. The cellular/mitochondrial function core provides expertise and access to core equipment that
can measure respiration and molecular aspects of cells and isolated mitochondria. Over the last grant period
the Molecular and Cellular Analytical Core has changed focus based on the demands of the NORC user base,
enhancing capabilities in lipidomics, mass spectrometry, and RNAseq bioinformatics and decreasing
capabilities in RT-PCR, ELISA, PI3kinase activity, and western blot analyses. The Molecular and Cellular
Analytical Core continues to provide a broad array of assays over a variety of tissue types (fat, muscle, liver,
bone), fluids (plasma, media, breast milk), and cells allowing for high throughput analysis for investigators
studying nutrition and obesity-related disorders. The Molecular and Cellular Analytical Core lab provides
NORC members with access to equipment, specific assays and services, and study and data consultation that
have helped to expand the quantity and quality of nutrition research in the NORC.

## Key facts

- **NIH application ID:** 10046142
- **Project number:** 2P30DK048520-26
- **Recipient organization:** UNIVERSITY OF COLORADO DENVER
- **Principal Investigator:** BRYAN C BERGMAN
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $233,250
- **Award type:** 2
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10046142

## Citation

> US National Institutes of Health, RePORTER application 10046142, Molecular and Cellular Analytical Core (2P30DK048520-26). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10046142. Licensed CC0.

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