# Xenopus Mutant Resource

> **NIH NIH R24** · MARINE BIOLOGICAL LABORATORY · 2020 · $793,571

## Abstract

Project Summary
This grant supports a project to generate and characterize Xenopus mutants in key genes related to human
disease, establish a new Xenopus Mutant Resource, develop new transgenic lines for genome editing and
establish efficient site-specific integration. The long-term goals of the studies are to generate new models of
human disease that will provide useful resources for the biomedical research community. The proposed
studies will use CRISPR-Cas gene editing to create precise models of human disease in the amphibian
Xenopus. The current studies are focused on developing Xenopus models of many different diseases for the
entire Xenopus community, and each mutant will be developed in close coordination with individual
researchers. There are four main aims to this proposal. First, we propose to collaborate with Xenopus
researchers to characterize existing mutants (over 116) that were developed over the last four years. The
mutants cover a wide range of topics that require varied expertise that can only be obtained through
interactions with other researchers. Second, we propose to generate new mutants using CRISPR-Cas as
requested by researchers. As the national stock center for Xenopus, we have the proven expertise to breed
and maintain these mutants. Third, we will generate new transgenic lines that will be used for genome editing.
These new lines will allow for more tissue-specific expression of Cas9. Fourth, we propose to develop
homology directed repair for more efficient generation of site-specific integration of exogenous DNA. All of
these aims will help enhance the utilization of CRISPR-Cas gene editing methods in the Xenopus model
system.

## Key facts

- **NIH application ID:** 10047403
- **Project number:** 1R24OD030008-01
- **Recipient organization:** MARINE BIOLOGICAL LABORATORY
- **Principal Investigator:** Marko E Horb
- **Activity code:** R24 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $793,571
- **Award type:** 1
- **Project period:** 2020-07-01 → 2024-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10047403

## Citation

> US National Institutes of Health, RePORTER application 10047403, Xenopus Mutant Resource (1R24OD030008-01). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10047403. Licensed CC0.

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