# Glycoproteomics in Ischemic Stroke Related Hyperglycemia

> **NIH NIH R21** · MASSACHUSETTS GENERAL HOSPITAL · 2020 · $462,000

## Abstract

Project Summary/Abstract
Acute hyperglycemia is a common clinical problem in ischemic stroke patients, and is frequently associated
with impaired thrombolytic efficacy of tPA and predictive of poor functional outcome of stroke patients.
However, the clinical management of acute hyperglycemia remains uncertain. Recent studies showed that
acute hyperglycemia is associated with rapid alteration in protein glycosylation, which is implicated in a wide
range of extracellular signaling pathways and biological functions. It is thus possible that acute hyperglycemia
dependent protein glycosylation might play a critical role in regulating thrombolysis and neurovascular injury in
stroke patients. We propose to use clinical proteomic approaches to examine protein glycosylation in these
patients and then probe mechanism in cell culture. The first aim is to systematically identify changes in blood
protein glycosylation associated with acute hyperglycemia in tPA-treated stroke patients using MS-based
approach to understand a broad landscape of clinical glyco-protein signals. Then, we validate the results in
blood samples of an independent, large cohort of ischemic stroke patients, to select novel biomarkers with high
translational impact. Finally, as brain microvascular endothelial cells play a critical role in regulating
thrombolysis and blood vessel function via synthesizing and secreting regulatory factors, we aim to examine
the glycosylation of proteins secreted by brain endothelial cells under hyperglycemic condition. This may help
to understand the role of brain endothelium in regulating extracellular response to ischemic stroke related
hyperglycemia. In this resubmission, we have revised extensively according to reviewers’ comments, including
adding new experts, new data and publications and experimental details and alternatives.

## Key facts

- **NIH application ID:** 10058002
- **Project number:** 1R21NS113065-01A1
- **Recipient organization:** MASSACHUSETTS GENERAL HOSPITAL
- **Principal Investigator:** MINGMING NING
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $462,000
- **Award type:** 1
- **Project period:** 2020-07-01 → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10058002

## Citation

> US National Institutes of Health, RePORTER application 10058002, Glycoproteomics in Ischemic Stroke Related Hyperglycemia (1R21NS113065-01A1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10058002. Licensed CC0.

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