# In Vivo Incorporation of Unnatural Amino Acids

> **NIH NIH R01** · SCRIPPS RESEARCH INSTITUTE, THE · 2021 · $498,263

## Abstract

Project Summary/Abstract
Our laboratory, and now many others, have added well over 100 noncanonical amino acids
(ncAAs) to the genetic codes of single and multi-cellular organisms [1, 2]. These amino acids
include spectroscopic probes, metal ion chelators, post-translationally modified amino acids and
stable analogues thereof, amino acids with bio-orthogonal chemical reactivity,
photocrosslinkers, and a host of other novel functions. Moreover, we have distributed this
technology to hundreds of laboratories throughout the world to probe protein structure and
function both inside and outside the cell, to engineer proteins with enhanced catalytic, physical
and biological properties, and to create novel biotherapeutics. Here we propose to further
expand our ability to manipulate the genetic code and apply this approach to important
problems in biomedical research including exploring the function of short open reading frame
encoded peptides, evolving proteins with enhanced properties, and genetically encoding ncAAs
in hematopoietic stem cells and lineages derived therefrom in mice.

## Key facts

- **NIH application ID:** 10061603
- **Project number:** 5R01GM062159-18
- **Recipient organization:** SCRIPPS RESEARCH INSTITUTE, THE
- **Principal Investigator:** PETER G SCHULTZ
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $498,263
- **Award type:** 5
- **Project period:** 2001-02-01 → 2022-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10061603

## Citation

> US National Institutes of Health, RePORTER application 10061603, In Vivo Incorporation of Unnatural Amino Acids (5R01GM062159-18). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10061603. Licensed CC0.

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