# The Role of Glial Cells in Chronic Pelvic Pain of Endometriosis

> **NIH NIH R21** · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · 2020 · $229,896

## Abstract

Program Director/Principal Investigator (Last, First, Middle): Nowak, Romana A.
Endometriosis is an inflammatory disease that affects 10% of women but is present in up to 70% of
women with pelvic pain and 30% of women with infertility. The estimated cost of endometriosis is over
$22 billion in the US alone, not counting associated chronic illnesses. Thus it is safe to say that
endometriosis is one of the costliest reproductive diseases in women not only in terms of treatment but
also lost productivity and quality of life in affected patients. The mechanisms involved in the
development and maintenance of persistent or chronic pain are not fully understood. One potential
mechanism is the process of ‘central sensitization’, whereby long lasting molecular changes promote
neuroplasticity of nociceptive neurons within the central nervous system (CNS) resulting in amplification
of pain signaling. The development of central sensitization involves changes to neuronal and glial cell
populations. Neural changes have been studied in endometriosis and it has been suggested that pain
attributed to endometriosis is likely to involve neuronal processes leading to central sensitization.
However, a potential role for glia has yet to be investigated. Our hypothesis is that mice with
endometriosis will show significant changes in the activation state and function of glial cells in the brain
and spinal cord in response to persistent pro-inflammatory signaling from activated macrophages in
ectopic lesions and peritoneal fluid and that this activation contributes to the development of chronic
visceral pain. The goal of this exploratory research proposal is to begin testing this hypothesis using an
in vivo mouse model of endometriosis to determine whether the presence of endometriotic lesions and
the subsequent activation of pro-inflammatory macrophages in the peritoneum and endometriotic
lesions causes substantial alterations in the proliferation, activation status and gene expression in glial
cells of the spinal cord and brain. We will test this hypothesis in two specific aims:
1): To characterize changes in activation and proliferation of glial cells in the brain and spinal
cord of mice that develop endometriosis and correlate these changes with development of
chronic visceral pain. This will be determined by comprehensive immunohistochemical analyses of
brains and spinal cords of mice with lesions as well as a panel of behavior tests that assess pain. 2):
To sequence and identify genome-wide transcriptional alterations in microglia of the brain and
spinal cord of mice with endometriosis. We will utilize single cell RNAseq (scRNAseq) technology
to analyze changes in gene expression over time in purified populations of glial cells in spinal cords
and brains of mice bearing endometriotic lesions and compare these changes with mice that have
undergone sham transplantation surgery.
PHS 398/2590 (Rev. 06/09) Page Continuation Format Page

## Key facts

- **NIH application ID:** 10062360
- **Project number:** 1R21HD100725-01A1
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
- **Principal Investigator:** Romana A. Nowak
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $229,896
- **Award type:** 1
- **Project period:** 2020-09-01 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10062360

## Citation

> US National Institutes of Health, RePORTER application 10062360, The Role of Glial Cells in Chronic Pelvic Pain of Endometriosis (1R21HD100725-01A1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10062360. Licensed CC0.

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