# Novel Enediyne-Based Antibody-Drug Conjugates for Cancers

> **NIH NIH R01** · SCRIPPS FLORIDA · 2021 · $535,980

## Abstract

Both the two FDA-approved antibody-drug conjugates (ADCs) (Adcetris® and Kadcyla®) and nearly all ADCs in
clinical trials are prepared by randomly conjugating drugs to lysine or cysteine residues in the antibody,
affording a heterogeneous ADC mixture. Site-specific conjugation is a major recent improvement to ADC
development, yielding homogeneous ADCs with greatly improved therapeutic index. Among the 60+ ADCs
currently in clinical development, nearly 50 of them use auristatin and maytansine, the two payloads used in
Adcetris® or Kadcyla®, respectively, with the rest using one of only five other drugs. The ADC field is in critical
need of new, highly potent, and rapidly acting cytotoxic payloads that are active in many tumor types. We
propose in this application to develop new enediyne-based site-specific ADCs for cancer therapy. Specifically,
we will focus on tiancimycins (TNMs), which we recently discovered as novel members of the enediyne family
of natural products. Our hypotheses are: (i) TNMs are outstanding ADC payload candidates owing to their
exquisite potency and validated mode of action, (ii) genetic manipulation of TNM biosynthesis provides
outstanding opportunities to produce the most promising TNM analogues to develop the linker chemistry and
facilitate site-specific conjugation, (iii) site-specific conjugation of TNMs to engineered thiomabs and
selenomabs will generate homogeneous ADCs with defined drug-to-antibody ratios (DARs), and (iv)
complementary targeting of HER2 and ROR1 will help evaluate the novel enediyne-based ADCs against
current benchmarks and improve therapeutic outcomes for breast cancer patients. The specific aims for this
grant are: (i) manipulation of TNM biosynthesis in Streptomyces sp. CB03234 to produce the most promising
TNMs for site-specific conjugation, (ii) production and purification of anti-HER2 and anti-ROR1 thiomabs and
selenomabs with 2 engineered cysteine (Cys) and 2 or 1 engineered selenocysteine (Sec) residues,
respectively, (iii) development of linker chemistry for site-specific conjugation and delivery of a panel of anti-
HER2 and anti-ROR1 thiomab-TNM and selenomab-TNM conjugates, and (iv) evaluation of selectivity and
potency of the anti-HER2 and anti-ROR1 thiomab-TNM and selenomab-TNM conjugates against HER2+ and
HER2−/ROR1+ breast cancers in both in vitro and in vivo models. The outcomes of this application include (i)
fundamental contributions to ADCs and (ii) a panel of anti-HER2 and anti-ROR1 thiomab-TNM and selenomab-
TNM conjugates as next-generation ADC therapeutics for cancers. The long-term goal of our research is to
discover novel microbial natural products and harness their exquisite cytotoxicity as ADC payloads for
anticancer drug discovery.

## Key facts

- **NIH application ID:** 10062881
- **Project number:** 5R01CA204484-05
- **Recipient organization:** SCRIPPS FLORIDA
- **Principal Investigator:** CHRISTOPH RADER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $535,980
- **Award type:** 5
- **Project period:** 2016-12-15 → 2022-04-01

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10062881

## Citation

> US National Institutes of Health, RePORTER application 10062881, Novel Enediyne-Based Antibody-Drug Conjugates for Cancers (5R01CA204484-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10062881. Licensed CC0.

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