# Ultra-Sensitive Multiplexed Quantification of Proteins Secreted from Single-Cells - Resubmission 01

> **NIH NIH R01** · UNIVERSITY OF CHICAGO · 2021 · $395,280

## Abstract

Ultra-Sensitive Multiplexed Quantification of Proteins Secreted from Single-Cells
This proposal aims to develop a powerful new technology for ultra-sensitive quantification of multiple proteins
secreted from single-cells. Secreted protein signals coordinate cellular and organism-level functions across
all biological systems, and their accurate quantification is crucial for understanding biological information
processing in general. Unfortunately, signaling studies have been hindered by poor sensitivity and limited
multiplexing ability of current proteomic methods, and the confusion of results due to pooling of multiple cell
readouts when conducting population-averaged assays. To address the need to quantify single-cell secreted
proteins across different signaling systems, we will develop a new microfluidic technology for ultra-sensitive,
multiplexed protein quantification at the single-cell level. We will use our wide-ranging expertise in single-cell
assay and microfluidic development and our extensive preliminary data to develop a user-friendly system
that will measure multiplexed protein signals from a wide range of cell types, in a time-dependent manner.
We will validate our method by quantifying signaling time courses of diverse systems including immune cells,
cancer secretion, and hormone signaling. This method will, for the first time, provide the means to gather
sensitive, multiplexed single-cell secreted protein measurements, and will be indispensable for
understanding signaling in immunity, metabolism, development and cancer.

## Key facts

- **NIH application ID:** 10070623
- **Project number:** 5R01GM127527-03
- **Recipient organization:** UNIVERSITY OF CHICAGO
- **Principal Investigator:** Savas Tay
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $395,280
- **Award type:** 5
- **Project period:** 2019-04-01 → 2022-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10070623

## Citation

> US National Institutes of Health, RePORTER application 10070623, Ultra-Sensitive Multiplexed Quantification of Proteins Secreted from Single-Cells - Resubmission 01 (5R01GM127527-03). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10070623. Licensed CC0.

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