# Core B:  Metabolism Core

> **NIH NIH P20** · UNIVERSITY OF KENTUCKY · 2021 · $245,689

## Abstract

PROJECT SUMMARY
 “Metabolomics” largely consists of metabolic profiling, which is the process of identification and
quantification of a large number of metabolites of many different biochemical classes, from diverse
biological samples, including cells, culture media, tissue and biofluids. However, to understand the
metabolic underpinning of diseases such as cancer, a biochemical mechanism is needed, in which
case it is necessary to introduce appropriate stable isotope tracers, which while increasing the
number of analytes to be quantified, also greatly increase the retrieval of biologically relevant
information. This Stable Isotope Resolved Metabolomics (SIRM) approach is a unique specialty of
the Metabolism Core. The goals of the Core are to provide services for a wide coverage of
metabolites with isotopomer and isotopologue analysis. To achieve this, we make use of two major
analytical platforms, namely high resolution nuclear magnetic resonance (NMR), and ultra-high
resolution Fourier-transform mass spectrometry (UHR-FTMS), with or without chromatography. The
Core personnel will advise on analytical methods and experimental design and additional techniques
may be developed to address issues of identification and quantification. Because experimental
design and sample preparation are integral to the data acquisition and interpretation, the Core
provides expertise in these and informatics relevant to the SIRM approach. These goals will be
achieved according to the following: Specific Aim 1. To provide access and expertise across a wide
range of NMR and MS technologies to investigators. Specific Aim 2. To provide experimental design,
sample processing, and informatics support to the investigators for data reduction, metabolic
pathway, and biochemical mechanism analyses. Specific Aim 3. To develop and implement new
methods to enhance metabolic capabilities relevant to the COBRE Projects. This Core will support
the young investigators by providing advanced analytical technologies and expertise in the metabolic
requirements for cancer development.

## Key facts

- **NIH application ID:** 10073529
- **Project number:** 5P20GM121327-05
- **Recipient organization:** UNIVERSITY OF KENTUCKY
- **Principal Investigator:** ANDREW N. LANE
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $245,689
- **Award type:** 5
- **Project period:** 2017-03-01 → 2021-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10073529

## Citation

> US National Institutes of Health, RePORTER application 10073529, Core B:  Metabolism Core (5P20GM121327-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10073529. Licensed CC0.

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