# Experimental endoscopic third ventriculostomy with choroid plexus cauterization and its effects on brain development

> **NIH NIH R21** · WASHINGTON UNIVERSITY · 2021 · $187,675

## Abstract

Hydrocephalus (HC) is a common and debilitating neurological condition affecting up to 1 in 500 individuals.
Ventriculoperitoneal shunting (VPS) has been the standard treatment for >60 years but shunts remain highly
problematic, with an unacceptably high malfunction rate and often lead to a lifetime of complex neurosurgical
care. Endoscopic third ventriculostomy (ETV) is an alternative to shunting but has limited efficacy in infants.
Adding choroid plexus cauterization (CPC) to ETV seems to be a viable alternative to shunting infants. Despite
conflicting outcomes, ETV-CPC is currently being offered routinely and globally, and with limited understanding
of the risks or benefits of this procedure on brain development. The lack of knowledge about both the surgical
and physiological consequences of ETV-CPC constitutes a significant barrier to broad acceptance of this
procedure. Experimental studies are needed to determine the role of ETV and ETV-CPC in clinical practice, but
the lack of appropriate, validated large animal models in which to test these techniques on brain physiology and,
critically, on brain development, has never been studied. For example, the impact of ablating the choroid plexus,
a major homeostatic organ which regulates neurogenesis and neurodevelopment, has yet to be determined. The
NIH has responded to the unmet need for appropriate animal models by initiating Funding Opportunity PA-18-
623, “Tools to Enhance the Study of Prenatal and Pediatric Hydrocephalus”, which promotes applications
designed “to transform the field of prenatal and/or pediatric hydrocephalus research by generating tools including
animal…models…to understand disease mechanisms and/or developing therapeutics”. Our needs-based and
hypothesis-driven proposal leverages our foundational work on ETV, ETV-CPC, and VPS to address the goals
of PA-180-623 with 3 Specific Aims: (1) Develop a clinically-relevant, large animal model of HC to compare
ETV, ETV-CPC, and VPS; (2) Define criteria for successful ETV, ETV-CPC, and VPS in the porcine model
and determine the effects of these procedures on periventricular microstructure and hippocampal
volume; and (3) Examine the effect of ETV, ETV-CPC, and VPS on cognitive function in piglets with infant
HC. Pigs were chosen for this model both for their close homology to human neuroanatomy and physiology and
the ability to use standard clinical neurosurgical techniques. Development of these novel treatment models of
infant HC will fill a critical void in HC research and enable rigorous testing of emerging surgical procedures and
injury mechanisms, which is essential to identifying best neurosurgical practices for the clinical management of
HC. The goal of PA-18-623 will be achieved because this model will be freely shared and implemented by other
investigators and educational programs.

## Key facts

- **NIH application ID:** 10073554
- **Project number:** 5R21NS111249-02
- **Recipient organization:** WASHINGTON UNIVERSITY
- **Principal Investigator:** David Delmar Limbrick
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $187,675
- **Award type:** 5
- **Project period:** 2020-01-01 → 2021-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10073554

## Citation

> US National Institutes of Health, RePORTER application 10073554, Experimental endoscopic third ventriculostomy with choroid plexus cauterization and its effects on brain development (5R21NS111249-02). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/10073554. Licensed CC0.

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