# Transcriptomics and Single Cell Analysis Core

> **NIH NIH U19** · COLUMBIA UNIVERSITY HEALTH SCIENCES · 2021 · $458,943

## Abstract

PROJECT SUMMARY
The main goal of this core is to establish and provide robust experimental procedures for profiling the
transcriptomes and proteomes of immune cells. We will provide innovative methods for molecular profiling in
bulk populations in the context of perturbations and at the single cell level for all of the proposed projects
including single cell RNA-Seq and mass cytometry by time-of-flight (CyTOF) for profiling single cell proteomes
and signaling. Projects in the Center demand population-level RNA-Seq on a relatively large-scale and involve
immune cell profiling for various tissue sites, in the context of different viral infections and perturbations, and at
different time points during perturbation experiments. To address these needs, the core will provide a novel
implementation of highly multiplexed RNA-Seq that allows large-scale, inexpensive expression profiling in an
automated, multi-well plate format. Single cell RNA-Seq and CyTOF are unique in their abilities to reveal
phenotypic heterogeneity among apparently homogeneous populations of cells. These capabilities are
essential for studying the dynamics of immune cells, which do not respond uniformly to perturbations and may
exhibit tissue-dependent phenotypes. Combining single cell RNA-Seq and CyTOF will reveal not only the
transcriptional heterogeneity underlying immune cell populations, but also protein markers and post-
translational modifications of signaling molecules like phosphorylation.

## Key facts

- **NIH application ID:** 10074526
- **Project number:** 5U19AI128949-05
- **Recipient organization:** COLUMBIA UNIVERSITY HEALTH SCIENCES
- **Principal Investigator:** Peter Alan Sims
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $458,943
- **Award type:** 5
- **Project period:** 2017-01-01 → 2022-03-17

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10074526

## Citation

> US National Institutes of Health, RePORTER application 10074526, Transcriptomics and Single Cell Analysis Core (5U19AI128949-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10074526. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*