# Coupling activation of primary sensory neurons as a novel plasticity mechanism for chronic pain

> **NIH NIH R01** · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · 2021 · $366,542

## Abstract

Chronic pain is affecting over 100 millions of U.S. adults with its debilitating medical problems, inadequate
treatment, and severe economic burdens (over $600 billion). This is due to limited efficacy or intolerable side
effects of available analgesic options. In order to better serve chronic pain patients, it is better to have clearer
understanding of how chronic pain is generated. We don’t know exactly how innocuous stimuli (i.e. normal
input to Aβ-fiber activation) become painful, leading to chronic pain after injury. And it remains unclear how the
encoding of mechanosensory information to brain area is conveyed after it is sensitized at the level of primary
sensory neuron and spinal cord. Although the mechanisms of hyperexcitability of individual DRG neurons have
been extensively studied, how DRG neurons function at a populational level as an ensemble under
physiological and pathological conditions is unclear due to the lack of suitable tools and techniques. Here, we
developed an imaging technique that allowed us to simultaneously monitor the activation of >1,600
neurons/DRG in response to mechanical stimulation applied to the skin in live mice. And we now acquired and
obtained mouse genetic tools that allow visualization of all four major Low Threshold Mechanoreceptor (LTMR)
subtypes; Aβ, SA-LTMRs, Aβ, RA-LTMRs, Aδ-LTMRs, and C-LTMRs. Specific labeling of distinct sensory
neuron subtypes provide an unprecedented opportunity to determine whether the activity of each subtype is
altered during chronic pain after nerve injury. Using this powerful techniques and tools, we discovered a
striking neuronal coupling phenomenon as a novel pain mechanism that adjacent neurons activate together
following inflammation or nerve injury, although this rarely happens in naïve animals. This is the first
demonstration that coupling phenomenon of primary sensory neurons after nerve injury contributes to new
mechanisms underlying chronic pain conditions. This coupling activation occurs among various sizes of
neurons including small-diameter nociceptors and large-diameter low-threshold mechanoreceptors. Combining
the imaging technique with pharmacological and genetic approaches, we found that the coupling is, in part,
mediated by an injury-induced upregulation of gap junction in satellite glial cells surrounding DRG neurons.
Blocking gap junctions significantly attenuated neuronal coupling in the DRG and also reduced mechanical
hyperalgesia. Therefore, neuronal coupling represents a new form of neuronal plasticity in the DRG and by
“hijacking” neighboring neurons through gap junction it contributes to pain hypersensitivity. Our studies suggest
a new strategy to alleviate neuropathic pain by inhibiting gap junction connection between DRG neurons.

## Key facts

- **NIH application ID:** 10078859
- **Project number:** 5R01DE026677-06
- **Recipient organization:** UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
- **Principal Investigator:** Yu Shin Kim
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $366,542
- **Award type:** 5
- **Project period:** 2019-09-01 → 2022-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10078859

## Citation

> US National Institutes of Health, RePORTER application 10078859, Coupling activation of primary sensory neurons as a novel plasticity mechanism for chronic pain (5R01DE026677-06). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10078859. Licensed CC0.

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