# Biomimetic cardiac patch capable of rapid angiogenesis

> **NIH NIH R01** · OHIO STATE UNIVERSITY · 2021 · $565,055

## Abstract

ABSTRACT
Heart failure is on the rise in epidemic global proportions affecting more than 23 million people worldwide
including 5.8 million individuals in the US alone. Acute myocardial infarction (MI) leading to ischemic
cardiomyopathy is the most common etiology for decreased ejection fraction heart failure. Cardiomyocytes
derived from the human inducible pluripotent stem cells (hiPSC-CMs) are promising as a novel autologous cell-
based therapy in heart disease. The current obstacles for cardiac regeneration using stem-cell based
therapies include cell survival and maturity, anisotropic structure and alignment of elongated cardiomyocytes,
electro-mechanical integration of the cardiac patch with the native myocardium, and rapid angiogenesis to
support cardiomyocytes in the regenerating myocardium. The main objective of this proposal is to develop a
thick mature and functional cardiac tissue that not only has the anisotropy of the native tissue but also
stimulates rapid angiogenesis (1-week). We hypothesize that a combination of a functional multi-layered
hiPSC-CMs cardiac patch paired with a bFGF scaffold significantly improves the early and late cardiomyocyte
survival and promotes rapid angiogenesis. This hypothesis will be tested in the following three specific aims;
Aim 1) This aim will determine the maturity, contractile function, and cell survivability of a hiPSC-CMs multi-
layered aligned nanofiber cardiac patch in vitro, Aim 2) This aim will establish the efficacy of bFGF releasing
scaffolds to enhance hiPSC-CMs survival and promote rapid angiogenesis in simulated ischemic conditions in
vitro, Aim 3) This aim will determine efficacy of the transplanted multi-layered hiPSC-CMs cardiac patch paired
with bFGF scaffold following myocardial infarction on cardiac function, cell engraftment, angiogenesis, tissue
oxygenation and electro-mechanical integration, in an in vivo rat model of MI. Overall, this proposal will
establish an innovative myocardial cell-based therapeutic strategy based on, (i) the combination of human
iPSC-derived terminal differentiated cardiomyocytes with a biodegradable aligned nanofiber scaffold, (ii) bFGF-
releasing scaffold to enhance early cell survival and rapid angiogenesis, and (iii) the non-invasive monitoring of
myocardial tissue oxygenation and cell engraftment in vivo. The outcome of this project will enable us to
develop a novel cardiac patch for translation into a large animal clinical model of MI for repairing the damaged
heart.

## Key facts

- **NIH application ID:** 10079400
- **Project number:** 5R01HL136232-05
- **Recipient organization:** OHIO STATE UNIVERSITY
- **Principal Investigator:** Mahmood Khan
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $565,055
- **Award type:** 5
- **Project period:** 2016-12-15 → 2023-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10079400

## Citation

> US National Institutes of Health, RePORTER application 10079400, Biomimetic cardiac patch capable of rapid angiogenesis (5R01HL136232-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10079400. Licensed CC0.

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