# Design and directed evolution of an 'Edmanase' enzyme for high-throughput peptide sequencing.

> **NIH NIH R44** · ENCODIA, INC. · 2020 · $749,718

## Abstract

ABSTRACT
More accessible and cost-effective technologies for analysis of the proteome would
accelerate biological and medical research. The goal of this proposal is to develop
enzymatic reagents to enable creation of a highly-scalable digital protein analysis
technology. The approach is broadly applicable and greatly improves upon cost,
sensitivity, usability, and throughput of current approaches.

## Key facts

- **NIH application ID:** 10080672
- **Project number:** 2R44GM130185-02
- **Recipient organization:** ENCODIA, INC.
- **Principal Investigator:** KEVIN L GUNDERSON
- **Activity code:** R44 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $749,718
- **Award type:** 2
- **Project period:** 2018-09-13 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10080672

## Citation

> US National Institutes of Health, RePORTER application 10080672, Design and directed evolution of an 'Edmanase' enzyme for high-throughput peptide sequencing. (2R44GM130185-02). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10080672. Licensed CC0.

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