# Integration of Wolbachia-based Incompatible Insect Technique (IIT) and Auto-Dissemination Augmented by Males (ADAM) for mosquito vector control

> **NIH NIH R43** · MOSQUITOMATE, INC. · 2020 · $193,500

## Abstract

Abstract
In the absence of effective, approved vaccines or therapeutic measures against dengue, Zika
and other mosquito borne pathogens, public health efforts must focus on the control of mosquito
vectors that are required for transmission. Unfortunately, effective control measures are not
available against invasive mosquito species like Aedes aegypti and Aedes albopictus, due in
part to problems resulting from their cryptic behaviour. Specifically, Aedes mosquitoes breed in
hidden/inaccessible sites that are sheltered from existing control methods, e.g., truck and aerial
spraying.
Autocidal technologies use the insect itself as a self-delivering control tool. As an example, the
Sterile Insect Technique (SIT) has been used successfully against multiple agricultural pests,
including large scale elimination from the North American continent (e.g., Screwworm;
Cochliomyia hominivorax). However, despite years of effort, SIT has not proven effective
against mosquitoes.
Through previous SBIR-funded research, MosquitoMate has developed two autocidal methods
against Ae. aegypti and Ae. albopictus mosquitoes, including: 1) Incompatible Insect Technique
(IIT) that uses a naturally occurring bacterium (Wolbachia) to sterilize populations, and 2)
Auto-Dissemination Augmented by Males (ADAM) that uses males as vehicles to spread a
potent insecticide. To date, the USA Environmental Protection Agency (EPA) has instructed
MosquitoMate to keep the IIT and ADAM approaches separate. In the work proposed here,
MosquitoMate will develop data that the EPA can consider for the integration of the two
approaches.
If awarded, the resulting research will examine parameters critical to the EPA’s efficacy and risk
assessments, including potential: 1) PPF effects on Wolbachia-induced sterility, 2) PPF effects
on male mosquito performance (e.g., ‘boosting’ of male competitiveness), and 3) PPF effects on
adult female mosquito fecundity and fitness. The resulting data will be communicated to the
EPA, including a pre-registration meeting to identify requirements leading to FIFRA Section 3
EPA pesticide registration for the combined IIT/ADAM approach.
Market potential exists at the local, corporate, government and area wide control levels for the
control of invasive, medically important mosquito species. MosquitoMate has an exclusive
license for the patented Wolbachia technology and additional patents on the ADAM technology.

## Key facts

- **NIH application ID:** 10081459
- **Project number:** 1R43AI155191-01
- **Recipient organization:** MOSQUITOMATE, INC.
- **Principal Investigator:** James William Mains
- **Activity code:** R43 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $193,500
- **Award type:** 1
- **Project period:** 2020-06-15 → 2022-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10081459

## Citation

> US National Institutes of Health, RePORTER application 10081459, Integration of Wolbachia-based Incompatible Insect Technique (IIT) and Auto-Dissemination Augmented by Males (ADAM) for mosquito vector control (1R43AI155191-01). Retrieved via AI Analytics 2026-06-01 from https://api.ai-analytics.org/grant/nih/10081459. Licensed CC0.

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