# Neuronal plasticity in retinal circuit development and disease

> **NIH NIH R01** · WASHINGTON UNIVERSITY · 2021 · $343,743

## Abstract

SUMMARY
Neuronal plasticity is high during development and low at maturity. High plasticity enables developing circuits to
refine their connections and attain specific functions, whereas low plasticity restricts rewiring in mature circuits,
limiting functional recovery from neurodegeneration and injury. Our proposal focuses on three questions: (1)
How do cell-type-specific plasticity mechanisms support the development of specific circuits? (2) What controls
the maturational switch from high to low plasticity? (3) How can we enhance plasticity of mature neurons to
promote functional recovery from neurodegeneration and injury? We will address these questions in retinal
bipolar cells. Bipolar cells are second-order neurons of the visual system and relay photoreceptor signals from
the outer retina to amacrine and ganglion cells in the inner retina. Bipolar cells lose input when photoreceptors
die in retinal degeneration, the most common heritable cause of visual impairment (i.e., > 1:2000 people
worldwide). Retinal degeneration, a heterogeneous group of diseases, often progresses slowly, leaving a window
of opportunity, in which rewiring of bipolar cells with remaining photoreceptors could rescue vision. In Aim 1 of
our proposal, we will characterize the developmental plasticity of three bipolar cell types, which participate in two
retinal circuits that support specific visually guided behaviors. Thus, we will link cell-type-specific plasticity
mechanisms to the development of specific circuits and the behaviors they support. In Aim 2, we will characterize
molecules and mechanisms that contribute to the maturational switch from high to low plasticity in the same
bipolar cells. We will translate insights into these molecules and mechanisms into viral tools to restore
developmental plasticity to mature neurons. In Aim 3, we will test the ability of these tools to rescue connectivity
and function in two mouse models of retinal degeneration. Throughout this proposal, we will use adeno-
associated viruses to manipulate plasticity. We will analyze bipolar cell morphology and connectivity by confocal
and superresolution imaging. We will monitor circuit function by two-photon Ca2+ imaging and patch clamp
electrophysiology, and we will test optokinetic responses and perceptual contrast sensitivity to assess visual
function of mice. Together, these studies will provide insights into the expression and control mechanisms of
plasticity and translate these insights into strategies to enhance plasticity of mature bipolar cells to rescue vision
during retinal degeneration.

## Key facts

- **NIH application ID:** 10085643
- **Project number:** 5R01EY023341-07
- **Recipient organization:** WASHINGTON UNIVERSITY
- **Principal Investigator:** Daniel Kerschensteiner
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $343,743
- **Award type:** 5
- **Project period:** 2014-01-01 → 2022-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10085643

## Citation

> US National Institutes of Health, RePORTER application 10085643, Neuronal plasticity in retinal circuit development and disease (5R01EY023341-07). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10085643. Licensed CC0.

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