# A Comprehensive Functional Map of Human Protein-RNA Interactions

> **NIH NIH U41** · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · 2021 · $756,571

## Abstract

PRODUCTION CORE – PROJECT SUMMARY
The objective of the ENCORE (Encyclopedia of RNA Elements) project is to develop a
foundational, functional map of protein-RNA interactions of RNA binding proteins
(RBPs) encoded in the human genome, and the RNA elements they bind to across the
transcriptome. These RNA elements, when expressed, form the basis of co- and post-
transcriptional regulation of human genes. Our strategy consists of developing and
integrating a physical map of 300 RBPs in two different human cell lines with
transcriptome-wide measurements of the effects of depleting these RBPs, their
localization patterns and their binding preferences independent of co-factor
associations. In this data production core, we will capitalize on the highly efficient data
production pipeline we have established over the past four years to generate RNA bind-
n-seq, RNA-seq, protein localization and eCLIP data for an additional 300 RBPs. When
combined with the data we generated over the past four years, these efforts will
culminate in a comprehensive map of the functional RNA elements recognized by
essentially all RBPs expressed in two human cell lines, representing approximately half
of the known complement of human RBPs. In summary, the data we will produce in this
project will enable a more systematic and comprehensive understanding of the role of
RBPs and RNA biology in the contribution to human biology and disease.

## Key facts

- **NIH application ID:** 10087952
- **Project number:** 5U41HG009889-04
- **Recipient organization:** UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
- **Principal Investigator:** Brenton R. Graveley
- **Activity code:** U41 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $756,571
- **Award type:** 5
- **Project period:** 2018-02-05 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10087952

## Citation

> US National Institutes of Health, RePORTER application 10087952, A Comprehensive Functional Map of Human Protein-RNA Interactions (5U41HG009889-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10087952. Licensed CC0.

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