# Core C: Imaging: from Super-Resolution to Whole Animal

> **NIH NIH P01** · COLD SPRING HARBOR LABORATORY · 2021 · $311,710

## Abstract

PROJECT SUMMARY – CORE C
Core C, which supports every project within the Program, provides access to sophisticated imaging
technologies and histopathology services, enabling Program investigators to use imaging to obtain insights into
molecular and cellular mechanisms and to track tumor progression, therapy response, and metastasis.
Imaging in cells and animals – from the molecular, to tissue, to whole-body level – is supported by the Core.
Core C will assist with sub-cellular to whole-tissue level analysis of fixed cells and tissues, providing
histopathological services through a subcontract with a world-class pathologist. The Core also supports live
cell imaging in three and four dimensions (3D and 4D, also known as 3D time-lapse), and imaging in live
animals. The imaging approaches offered by Core C include several cutting-edge imaging technologies, each
of which is available in a limited number of locations worldwide. These include i) super-resolution (100 nm),
and high-speed cellular imaging achieved with an OMX (Optical Microscope eXperimental) microscope; ii) 3D
whole-tissue reconstruction of tumors and metastases using light-sheet microscopy; and iii) intravital (live
animal) imaging using custom-built spinning disk confocal and multi-photon microscopes.
During the upcoming period of requested support, Core C proposes to support Program investigators through
four aims. First, the program will provide assistance with histology, immune labeling, and histopathology.
Second, the Core will support live cell imaging and super-resolution imaging. Third, the Core will assist with
imaging at the tissue level, either using 3D tissue reconstruction or live imaging in animals. These technologies
each require custom-built microscopes that are not generally available. Fourth, to provide Program
investigators with tools to track tumor growth and metastasis, Core C will assist with whole animal imaging
approaches, including bioluminescence, fluorescent proteins, and small animal ultrasound. Core C will also
assist with the use of advanced imaging software, including Image J (NIH), Imaris (Bitplane), and Volocity
(Perkin-Elmer). New in this renewal is support for quantification of micrometastasis, intravital imaging through
surgically implanted windows, and 3D tissue reconstruction using light-sheet microscopy.
Together, Core C has an unprecedented imaging platform, covering super-resolution imaging, 3D tissue
reconstruction, live cell tracking in tissues and whole animal imaging. A Core that enables program
investigators access to these technologies is essential as most of the imaging technologies supported by the
Core are highly sophisticated and require very specialized training. Furthermore, access to the instruments
required for these imaging technologies would be cost-prohibitive without the support of a Core. Thus, Core C
will provide critical tools for Program members and, through the development of new imaging approaches,
ultimately the broader c...

## Key facts

- **NIH application ID:** 10092145
- **Project number:** 5P01CA013106-49
- **Recipient organization:** COLD SPRING HARBOR LABORATORY
- **Principal Investigator:** Mikala Egeblad
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $311,710
- **Award type:** 5
- **Project period:** 1997-02-10 → 2023-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10092145

## Citation

> US National Institutes of Health, RePORTER application 10092145, Core C: Imaging: from Super-Resolution to Whole Animal (5P01CA013106-49). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10092145. Licensed CC0.

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