# Demonstrate the molecular receptor and functions of dorsal organ warm cells in flies

> **NIH NIH R01** · VIRGINIA POLYTECHNIC INST AND ST UNIV · 2021 · $336,000

## Abstract

Summary – Lina Ni
 Animals depend on their temperature-sensing systems to avoid noxious thermal extremes and to seek
optimal temperatures for survival. Temperature sensation is particularly relevant for small animals, such as
insects, which rely on ambient temperatures to set their body temperatures. Many insect vectors of diseases,
such as mosquitoes, respond to the temperature of their warm-blooded hosts and use it to guide the blood-
feeding behaviors through which they transmit human diseases. Thus, it is important to identify the temperature-
sensing molecules and neurons to help control disease vectors. Fruit flies are a suitable model system for these
studies, because of their evolutionarily conserved temperature-sensing molecules with mosquito. In flies, many
temperature-sensing systems possess a small number of temperature sensory neurons that master robust
behaviors. Moreover, the powerful genetics developed in flies ensures the precise manipulation of temperature-
sensing molecules and neurons. Preliminary studies from the PI’s lab discovered a set of previously unidentified
warm-activated neurons in fly larvae, whose temperature-sensing molecules and functions are unknown.
Preliminary data suggested that these neurons depended on a new class of warm-sensing molecules to detect
warm temperatures. Besides a classic function of temperature-responsive neurons that determine temperature
preference, these neurons may have an additional function to modulate their neighboring cool-activated neurons.
Such modulation may result in change of physiological properties in neighboring neurons. The goal of this
application is to identify the warm-sensing molecules in these warm-activated neurons, to demonstrate their
functions in temperature preference, and to determine their modulatory effects on neighboring neurons. In
addition to having preliminary data, we are particularly well-prepared to undertake the proposed research
because of our extensive, and successful, track record of studying temperature-responsive molecules and
sensory neurons in flies. Since the potential candidates of the warm-sensing molecules are conserved between
flies and mosquitoes, this study might provide novel targets to control blood-feeding behaviors of mosquitoes
and other disease vectors.

## Key facts

- **NIH application ID:** 10098871
- **Project number:** 1R01GM140130-01
- **Recipient organization:** VIRGINIA POLYTECHNIC INST AND ST UNIV
- **Principal Investigator:** Lina Ni
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $336,000
- **Award type:** 1
- **Project period:** 2020-12-01 → 2025-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10098871

## Citation

> US National Institutes of Health, RePORTER application 10098871, Demonstrate the molecular receptor and functions of dorsal organ warm cells in flies (1R01GM140130-01). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10098871. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*