# Development and Application of Non-Fourier Method for Biomolecular NMR

> **NIH NIH R01** · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · 2021 · $319,000

## Abstract

Project Summary
Non-Fourier methods of spectrum analysis have enabled previously intractable multidimensional NMR
experiment on biomolecules by providing high resolution spectra from data collected using nonuniform (sparse)
sampling. Compared to the Fourier Transform, we know much less about the properties of these methods. In
order to fully realize their potential benefits, and to understand their vulnerabilities, it is essential that we
investigate their robustness, failure modes, and convergence properties. Further improvements in these
methods are essential for meeting the challenges posed by larger, more complex, fleetingly stable, and
dynamically disordered systems, but the lack of detailed understanding as well as the absence of robust
metrics for spectral quality has hampered systematic approaches, critical comparison of competing
approaches, or determination of best practices. The broad aim of this proposal is to develop robust metrics and
apply them to improve the methods, and apply them to enable challenging biomolecular applications of
multidimensional NMR. A domain of the protein USP7, involved in cancer, will serve as a case study. The
potential impact of these developments is substantial because they will improve the power of the installed base
of NMR spectrometers for biomolecular applications.

## Key facts

- **NIH application ID:** 10101656
- **Project number:** 5R01GM123249-04
- **Recipient organization:** UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
- **Principal Investigator:** JEFFREY C HOCH
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $319,000
- **Award type:** 5
- **Project period:** 2018-02-01 → 2023-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10101656

## Citation

> US National Institutes of Health, RePORTER application 10101656, Development and Application of Non-Fourier Method for Biomolecular NMR (5R01GM123249-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10101656. Licensed CC0.

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