# Tissue-specific protein interactome mapping in a vertebrate embryo

> **NIH NIH R21** · UNIVERSITY OF TEXAS AT AUSTIN · 2020 · $237,750

## Abstract

Abstract
Proteins rarely act in isolation, but rather function in multi-protein complexes. Accordingly, protein-protein
interactomes are exceptionally valuable resources that provide deep mechanistic insights and generate myriad
hypotheses. Current methods for interactome mapping, such as affinity purification mass-spectrometry
(APMS), are extremely difficult to deploy in vivo, so little comprehensive interactome data yet exists for
developing embryos and even less for specific tissues within embryos. This fact poses an especially acute
problem for understanding highly dynamic processes in which post-transcriptional controls dominate, for
example collective cell movements. Here, we will use tissue engaged in convergent extension, a crucial
collective movement that elongates the axis of animal embryos, to test the efficacy of new label-free
interactome mapping approaches. Successful completion of the project will therefore be significant both for
developing broadly applicable new methods and also for providing systems-level insights into a disease-
relevant, vertebrate collective cell movement.

## Key facts

- **NIH application ID:** 10104048
- **Project number:** 1R21HD103882-01
- **Recipient organization:** UNIVERSITY OF TEXAS AT AUSTIN
- **Principal Investigator:** EDWARD M MARCOTTE
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $237,750
- **Award type:** 1
- **Project period:** 2020-09-25 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10104048

## Citation

> US National Institutes of Health, RePORTER application 10104048, Tissue-specific protein interactome mapping in a vertebrate embryo (1R21HD103882-01). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10104048. Licensed CC0.

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