# Clinical Metabolism Core

> **NIH NIH P30** · YALE UNIVERSITY · 2021 · $243,401

## Abstract

The Yale-DRC Clinical Metabolism Core provides comprehensive support for investigators conducting clinical
investigations of human diseases of metabolism such as diabetes. The primary emphasis of this core is to
provide analytical resources for patient-oriented studies utilizing stable isotopes to determine metabolic flux at
the whole body and tissue specific levels. Secondarily, the core also makes its analytical resources available to
researchers utilizing rat and cell models of human metabolic diseases. Stable isotopes offer unique advantages
over traditional radioisotopic methods for assessing substrate turnover in humans as they do not expose subjects
to ioniziing radiation and they provide positional isotopomer information that can be used to assess flux through
critical metabolic pathways. The major limitation to the use of stable isotopes by the clinical investigator is the
need for sophisticated and expensive instrumentation and highly skilled expertise for instrument operation and
for data analysis and interpretation. The Yale-DRC Clinical Metabolism Core removes these obstacles by
providing the personnel and resources needed for the extraction, purification, derivatization, and instrumental
analysis needed to determine the concentrations and isotopic enrichments of metabolites in plasm a, urine, or
tissues. This core measures the isotopic (e.g., 2H, 13C, 15N, and 18O) enrichment and concentrations of over 140
intermediary metabolites by GC-MS, LC/MS/MS, and NMR for the calculation of turnover of carbohydrates, lipids,
and proteins. The primary purposes of the Yale-DRC Clinical Metabolism Core are to: 1) make GC-MS, LC-
MS/MS, and NMR analyses available to Yale DRC members, 2) avoid duplication of costs associated with
personnel and instrumentation, 3.) provide standardized protocols to insure consistent and accurate sample
analysis, 4) assist Yale DRC researchers in the design and interpretation of experiments utilizing stable isotopes
for measurement of metabolic flux, 5.) develop new methodology in response to the emerging research needs
of Yale DRC members.

## Key facts

- **NIH application ID:** 10104494
- **Project number:** 5P30DK045735-29
- **Recipient organization:** YALE UNIVERSITY
- **Principal Investigator:** GERALD I SHULMAN
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $243,401
- **Award type:** 5
- **Project period:** 1997-01-01 → 2023-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10104494

## Citation

> US National Institutes of Health, RePORTER application 10104494, Clinical Metabolism Core (5P30DK045735-29). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10104494. Licensed CC0.

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