# Research Project #3

> **NIH NIH U42** · UNIVERSITY OF CALIFORNIA AT DAVIS · 2021 · $75,377

## Abstract

ABSTRACT: Project 3, Multi-omic analysis to expand phenotype in MMRRC mice
Established by the NIH 20 years ago, the MMRRC serves the biomedical research community by preserving,
protecting, and promoting the considerable national investment in creating and phenotyping mutant mouse
models for the study of human biology and disease. With a census today exceeding 60,000 alleles, the
MMRRC ensures that unique and scientifically valuable mouse strains produced and studied in independent PI
laboratories can be deposited and archived for safekeeping so that they are available and accessible in
perpetuity for scientists to obtain and use for their research studies. But the phenotypic description of mutant
mice submitted to the MMRRC often narrowly reflects the specific scientific interests of the submitting
investigator, reflecting the focus of the researcher who developed the line. Therefore, the phenotypic repertoire
of many (if not most) MMRRC lines is far more diverse than what is known. For that reason, anything the
MMRRC can do to expose these hidden phenotypes will at least add new knowledge about a line, some (if not
most) of which will be scientifically valuable. Therefore, this project will pilot the feasibility of conducting
relatively high-throughput, low cost, and unbiased phenotyping analyses to each mouse line submitted to the
MMRRC. To that end, we propose to use extant, in-house transcriptomic, proteomic, and metabolomic
platforms to establish a reliable and cost-effective protocol for high-fidelity multi-omic analysis of mouse tissues
from MMRRC mice. We will conduct this study in two aims over the course of 5 years. The first aim will
establish and test procedures in wildtype mice, and in the second aim apply the protocol to reveal new
phenotypes in MMRRC strains. We expect that comprehensive multi-omic characterization of submitted mouse
lines will not only contribute to validating what is already known (i.e., reproducibility and reliability) but also will
add an abundance of new phenotypic knowledge about MMRRC mouse lines. With this additional molecular
information, the research utility of mutant mouse lines held by the MMRRC Consortium will increase, resulting
in a growth in requests and greater use by the research community, and enhancing the distribution activity of
the resource.

## Key facts

- **NIH application ID:** 10104556
- **Project number:** 5U42OD012210-22
- **Recipient organization:** UNIVERSITY OF CALIFORNIA AT DAVIS
- **Principal Investigator:** KC KENT LLOYD
- **Activity code:** U42 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $75,377
- **Award type:** 5
- **Project period:** 1999-09-30 → 2025-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10104556

## Citation

> US National Institutes of Health, RePORTER application 10104556, Research Project #3 (5U42OD012210-22). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10104556. Licensed CC0.

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