# Regulation of Cardiac Calcium Transport

> **NIH NIH R01** · LOYOLA UNIVERSITY CHICAGO · 2021 · $488,949

## Abstract

7. Project Summary/Abstract
The applicant proposes a two aim strategy to explore molecular mechanisms of the
regulation of ion-motive ATPases. Aim 1 focuses on the mechanism of SERCA
regulation by phospholamban. This aim will determine how calcium relieves inhibition
of SERCA by phospholamban and test the novel hypothesis that there is an undiscovered
second binding site on SERCA for phospholamban. The planned experiments will also
reveal how phosphorylation alters the localization of the phospholamban cytoplasmic
domain on the SERCA cytoplasmic headpiece. There is still no high resolution structural
information relating this regulatory mechanism; it is most important unanswered question
in the field. Aim 2 will focus on the structural changes of the SERCA pump during the
transport cycle. Protein structural transitions will be quantified with advanced
fluorescence methods and molecular dynamics simulations. The experiments will
provide information about how the physical motions of pumps are important for their
biological function.

## Key facts

- **NIH application ID:** 10109137
- **Project number:** 5R01HL092321-13
- **Recipient organization:** LOYOLA UNIVERSITY CHICAGO
- **Principal Investigator:** Seth L Robia
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $488,949
- **Award type:** 5
- **Project period:** 2008-07-01 → 2022-07-24

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10109137

## Citation

> US National Institutes of Health, RePORTER application 10109137, Regulation of Cardiac Calcium Transport (5R01HL092321-13). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10109137. Licensed CC0.

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