# Shared Resource: Transgenic Mouse Facility

> **NIH NIH P30** · RESEARCH INST OF FOX CHASE CAN CTR · 2020 · $8,695

## Abstract

MODEL SYSTEMS SHARED RESOURCE: TRANSGENIC MOUSE FACILITY (TMF)
PROJECT SUMMARY/ABSTRACT
The Transgenic Mouse Facility (TMF) provides Cancer Center members the ability to introduce manipulated
genes and delete or modify endogenous genes in the germline of animals for Cancer Center members. This is
a major technological advance in biology, enabling investigators to answer questions about gene function,
which often must be analyzed in a whole animal model system. The TMF under the direction of Dietmar
Kappes, PhD (BCDF) and 2.0 FTE staff have provided knockout animals that have been instrumental in
providing insights into mechanisms of developmental gene regulation, cellular interactions within the immune
system, and the effect of oncogenes on growth and differentiation. 22 Cancer Center members used the TMF
in calendar year 2014. 95% of TMF users during 2014 were supported by peer-reviewed funding. 29 Cancer
Center members from all 5 Research Programs used the TMF in the period 2011-2014. Additionally, TMF
services are available to investigators at the Wistar Institute and Thomas Jefferson University (TJU). Since
2012, the TMF has provided service to 6 peer-review funded investigators from Wistar, and 2 peer reviewed
funded investigators from TJU. Kappes' years of experience and expertise is key in implementing state-of-the-
art technology and innovative approaches. Under Kappes' leadership coupled with institutional investment of
$81,498 TMF capabilities were improved by purchasing a new inverted microscope with Differential
Interference Contrast (DIC) optics, new micromanipulators for handling and injecting embryos, and a Hamilton
Thorne laser drilling system. The TMF has utilized zinc finger nuclease (ZFN)-mediated gene targeting
extensively and has recently added and validated the capacity to use CRISPR-mediated gene targeting. These
approaches considerably accelerate and simplify the generation of knockout and knock-in mice, and allow
generation of complex mouse models with multiple genetic alterations that were not previously feasible. Due to
the complexity of the services provided and the cost and caliber of the specialized instrumentation, these
services are best facilitated though the centralized TMF. Additional value added is that all Transgenic and
Knockout Projects were successful in 2014, resulting in several lines of each desired transgenic or knockout
strain. The Facility's 100% success rate attests to its high technical standards and rigorous quality control
procedures. Cryopreservation services offered by the TMF provides valuable insurance against loss of
irreplaceable mouse lines. The TMF is advised by a dedicated Facility Advisory Committee (FAC) that meets
annually. Recommendations from the FAC are directed to a governing Facility Parent Oversight Committee
that serves to ensure that the TMF continues to have the necessary resources to provide the highest quality
services for Cancer Center members. Demand for services is expect...

## Key facts

- **NIH application ID:** 10113013
- **Project number:** 3P30CA006927-54S4
- **Recipient organization:** RESEARCH INST OF FOX CHASE CAN CTR
- **Principal Investigator:** Dietmar J Kappes
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $8,695
- **Award type:** 3
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10113013

## Citation

> US National Institutes of Health, RePORTER application 10113013, Shared Resource: Transgenic Mouse Facility (3P30CA006927-54S4). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10113013. Licensed CC0.

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