# Microscopy & Cell Analysis Shared Resource

> **NIH NIH P30** · MAYO CLINIC ROCHESTER · 2021 · $147,062

## Abstract

MICROSCOPY AND CELL ANALYSIS SHARED RESOURCE PROJECT SUMMARY
Decoding mechanisms in cancer biology requires a fundamental understanding of the relationship between
structure and function. This is especially important for assessing tissue/tumor cell context, and at the
macomolecular level, for organelle function and integrity, and chromosome structure and behavior. The
Microscopy and Cell Analysis Shared Resource (MIC) provides exceptionally maintained high-end equipment
for optical and electron microscopy, cytometry and cell sorting, and provides the technical expertise for their
use to Mayo Clinic Cancer Center (MCCC) members conducting clinical or basic science cancer research. This
shared resource is heavily used with 161 MCCC members using the facility in 2017, from each of the MCCC
sites. The facility operates 10 hours/day on weekdays and 6 hours/day on weekends for assisted use, and for
most areas 24/7 for unassisted use. The MIC provides access, training, and expertise for a wide array of state-
of-the-art light and electron microscopy instrumentation, and for cell sorting and analysis. Our portfolio of
instrumentation allows MCCC members to image single molecules, in live cells and tissues, or fixed specimens
at optical or electron microscopic resolution, to analyze image data files by a wide variety of image analysis
and 3D reconstruction packages, and to perform multi-color flow analysis and sorting of fixed or live cells. We
support advanced optical imaging techniques such as FRET, TIRF, PALM, multi-photon, and 3D serial electron
microscopy, as well as state-of-the-art flow cytometry and cell sorting instrumentation and analysis methods.
The facility has aggressively pursued instrumentation funding which has allowed us to recently obtain a Zeiss
Elyra super-resolution microscope, and this past year, a FEI Apreo Serial Block Face Electron Microscope.
The MIC also provides cyberinfrastructure for data analysis, transfer, and storage of extremely large image
data sets.

## Key facts

- **NIH application ID:** 10113576
- **Project number:** 5P30CA015083-47
- **Recipient organization:** MAYO CLINIC ROCHESTER
- **Principal Investigator:** JEFFREY L SALISBURY
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $147,062
- **Award type:** 5
- **Project period:** 1997-04-25 → 2024-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10113576

## Citation

> US National Institutes of Health, RePORTER application 10113576, Microscopy & Cell Analysis Shared Resource (5P30CA015083-47). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10113576. Licensed CC0.

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