# Bioprinting Patterning for Cell-Laden Constructs

> **NIH NIH P41** · UNIV OF MARYLAND, COLLEGE PARK · 2021 · $265,824

## Abstract

TR&D 2: Bioprinting Patterning for Cell-Laden Constructs 
Project Summary 
A major challenge for tissue and organ engineering is the production of three-dimensional (3D) biomimetic, 
cellular tissue constructs of clinically relevant size, shape, and structural integrity needed for the replacement of 
damaged or injured tissues. To address this need, we have been investigating 3D bioprinting technologies, which 
are designed to print cell-laden hydrogel bioinks as well as polymeric biomaterials, to manufacture complex, 
multi-cellular living tissue constructs that mimic the structure of native tissues. In addition, we have been 
optimizing the formulation of biomaterials to serve as the scaffolding for 3D bioprinting, and providing the 
biological environment needed for the successful delivery of cells and biomaterials to specific locations within 
the 3D structures. It has become evident that the patterning design of the cell-laden biomaterials is critical for 
achieving adequate nutrition and functional outcomes. In this project, we will (1) develop bioink formulations and 
functionalize to control cell positioning with high printability, (2) fabricate biofunctional bioink formulations to 
control the cell microenvironment, and (3) validate bioprinted multiple cell populations with interacting 
functionalities for bone tissue regeneration in vivo. The results of this project - which will be shared with the broad 
community - will be to establish the ideal parameters needed for cell positioning during 3D printing of tissues, 
thus establishing a community wide approach for engineering tissues.

## Key facts

- **NIH application ID:** 10113635
- **Project number:** 5P41EB023833-05
- **Recipient organization:** UNIV OF MARYLAND, COLLEGE PARK
- **Principal Investigator:** ANTHONY ATALA
- **Activity code:** P41 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $265,824
- **Award type:** 5
- **Project period:** 2017-04-15 → 2023-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10113635

## Citation

> US National Institutes of Health, RePORTER application 10113635, Bioprinting Patterning for Cell-Laden Constructs (5P41EB023833-05). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/10113635. Licensed CC0.

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