# Single Cell Spatial Characterization of the Human Bone Marrow Microenvironment

> **NIH NIH R21** · WASHINGTON UNIVERSITY · 2021 · $196,875

## Abstract

Project Summary/Abstract
The objective of this project is to utilize high dimensional single cell approaches to delineate the spatial
architecture of human bone marrow. Functions of hematopoietic stems cells are known to be modulated by
signals generated by stromal cells in the bone marrow. Geographic relationships between hematopoietic cells
and specific stromal cell populations are thought to be a critical component of this interaction. While studies in
animal models have elucidated many of these key relationships, similar studies in human bone marrow have
been limited. We therefore propose to utilize imaging mass cytometry (IMC) to directly interrogate the cellular
architecture of the human bone marrow microenvironment. We will couple this approach with single cell RNA
sequencing to enable the identification of novel markers of specific stromal cell populations. These findings will
be leveraged to further inform the IMC approach. Within this context, we will develop a road map of normal
human bone marrow, which can serve as the framework for subsequent studies of both normal and diseased
hematopoietic states.

## Key facts

- **NIH application ID:** 10115110
- **Project number:** 5R21HL150636-02
- **Recipient organization:** WASHINGTON UNIVERSITY
- **Principal Investigator:** Daniel C Link
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $196,875
- **Award type:** 5
- **Project period:** 2020-03-01 → 2022-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10115110

## Citation

> US National Institutes of Health, RePORTER application 10115110, Single Cell Spatial Characterization of the Human Bone Marrow Microenvironment (5R21HL150636-02). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10115110. Licensed CC0.

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