# Cell and Tissue Imaging Shared Resources Core

> **NIH NIH P30** · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · 2021 · $321,434

## Abstract

PROJECT SUMMARY/ABSTRACT—Cell and Tissue Imaging Shared Resource 
The primary goal of the Cell and Tissue Imaging Shared Resource (CTISR) is to make state-of-the-art technology 
and analysis tools for imaging cells and tissues available to SJCCC investigators and trainees. The CTISR 
consists of 2 divisions: Light Microscopy, led by Dr. Victoria Frohlich, and Electron Microscopy, led by Sharon 
Frase. Together, Frohlich and Frase have more than 90 years of experience in scientific imaging. The CTISR is 
further staffed by 8 experienced imaging scientists who assist with acquisition, analysis, quantification, 
presentation, and publication of imaging data. The CTISR staff collaborates with SJCCC members prior to 
beginning a project to provide guidance on experimental design, appropriate imaging modalities, sample 
preparation, and data-analysis options. The degree of direct staff involvement in specimen preparation, imaging, 
and analysis is determined. The CTISR also provides user training when applicable. During the reporting period 
(FY2017), CTISR-LM conducted 60 consultations with 40 SJCCC labs and trained 95 new instrument users. 
Selected accomplishments in the current reporting period include a collaboration with Drs. Tanja Mittag (CBP), 
Richard Kriwacki (CBP), and J. Paul Taylor (NBTP) to visualize and characterize the role of liquid–liquid phase 
transition in the formation and maintenance of membraneless organelles. This work is now being expanded in 
an SJCCC-funded inter-center Collaborative as described in the Strategic Plan section of the Director's 
Overview. Additionally, CTISR staff assisted Dr. Charles Mullighan (HMP Co-Leader) to document, by confocal 
and in situ multiphoton imaging, the perturbations and stem cell-like phenotype caused by mutated IKZF1, a 
hallmark of high-risk acute lymphoblastic leukemia (ALL) and to detect the reversal by treatment with retinoids. 
As a result, clinical evaluation of retinoids for IKZF1-altered Philadelphia chromosome-positive ALL is being 
pursued (Churchman et al., Cancer Cell, 2015). During the current project period, CTISR contributed to 159 
publications authored by SJCCC members. Of these, 40 (25%) were interprogrammatic and 34 (21%) were 
intraprogrammatic. CTISR staff were co-authors of 24 (15%) of the publications, signifying the value of their 
expertise to the research presented. CTISR resources contributed to 70 publications in high-impact journals 
(impact factor >10) such as Cell, Nature, and Nature Genetics. Over the next five years, the CTISR plans to 
continuously expand and evolve services—including education and training programs—and technologies such 
as Lattice Lightsheet and virtual reality visualization, enabling SJCCC members to effectively pursue emerging 
directions in cancer research. CTISR staff will continue to educate SJCCC members on available methods to 
use correlative microscopy (CLEM) approaches to enhance cancer research. By leveraging and incorporatin...

## Key facts

- **NIH application ID:** 10116301
- **Project number:** 5P30CA021765-42
- **Recipient organization:** ST. JUDE CHILDREN'S RESEARCH HOSPITAL
- **Principal Investigator:** CAMENZIND G ROBINSON
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $321,434
- **Award type:** 5
- **Project period:** 1997-04-01 → 2024-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10116301

## Citation

> US National Institutes of Health, RePORTER application 10116301, Cell and Tissue Imaging Shared Resources Core (5P30CA021765-42). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10116301. Licensed CC0.

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