# The functions of the zinc finger protein ZPR1 in R-loop metabolism and neurodegeneration.

> **NIH NIH R01** · TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER AT EL PASO · 2021 · $444,825

## Abstract

PROJECT SUMMARY
The goal of this study is to examine the functions of the zinc finger protein ZPR1 in R-loops metabolism and
neurodegeneration. R-loops are formed during transcription and consist of RNA-DNA hybridized strands and a
complementary DNA strand. R-loop accumulation results in DNA damage leading to neurodegeneration
associated with genetic neurodegenerative diseases, including spinal muscular atrophy (SMA) and amyotrophic
lateral sclerosis (ALS). ZPR1 is evolutionary conserved in eukaryotes and is essential for cell viability. However,
very little is known about the ZPR1 biological functions that may contribute to cell viability and human disease
etiology. ZPR1 interacts directly with translation elongation factor 1A (EF1A), RNA Polymerase II and Senataxin
(SETX), which are also conserved in eukaryotes. SETX is an RNA-DNA helicase required for resolution of R-
loops. Mutations in SETX are associated with a group of untreatable neurodegenerative disorders, including
ataxia oculomotor apraxia type 2, autosomal dominant SMA and ALS4, characterized by defects in R-loop
metabolism. The molecular mechanisms of R-loop resolution are largely unknown. Our preliminary data show
that ZPR1 deficiency causes R-loop accumulation and neurodegeneration. ZPR1 overexpression reduces R-
loops and rescues DNA damage in neurons and patient cells, and prevents neurodegeneration in SMA mice.
ZPR1 binds to RNA-DNA hybrids and associates with R-loops in vivo. ZPR1 interacts with SETX and ZPR1 is
required for the formation of SETX complexes with R-loops suggesting that ZPR1 may help recruit SETX to R-
loops. We have created novel Zpr1 mutant mice, with double and quadruple point mutations in the Zpr1 locus to
selectively disrupt ZPR1-EF1A complexes. ZPR1 mutant mice show accumulation of R-loops and develop
neurodegenerative disease-like phenotypes similar to reported for patients with SETX mutations. Together,
these findings raise a hypothesis that ZPR1 complexes with EF1A and SETX may play distinct and critical roles
in R-loop resolution and provide a foundation for investigating the function of ZPR1-EF1A and ZPR1-SETX
complexes in R-loop metabolism. The specific aims are to examine: (Aim 1) the molecular basis of ZPR1-
dependent accumulation of co-transcriptional R-loops and neurodegeneration using Zpr1 conditional mice; (Aim
2) the function of ZPR1-EF1A complexes in R-loop resolution using novel mouse models to disrupt ZPR1-EF1A
complexes in vivo in motor neurons that we have generated, and the mechanism of GTP/GDP-dependent
resolution of RNA and DNA strands by ZPR1-EF1A complexes; (Aim 3) the function of ZPR1-SETX complexes
in R-loop metabolism, genome integrity and ALS4 pathogenesis. ZPR1-SETX complexes are disrupted in ALS4
patients with SETX mutation. The effect of disruption of ZPR1-SETX complexes on R-loop metabolism, DNA
replication fork and genome integrity using cell-based models, including patient cells. This study will provide
comprehensive insigh...

## Key facts

- **NIH application ID:** 10119365
- **Project number:** 1R01NS115834-01A1
- **Recipient organization:** TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER AT EL PASO
- **Principal Investigator:** Laxman Dass Gangwani
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $444,825
- **Award type:** 1
- **Project period:** 2021-01-01 → 2025-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10119365

## Citation

> US National Institutes of Health, RePORTER application 10119365, The functions of the zinc finger protein ZPR1 in R-loop metabolism and neurodegeneration. (1R01NS115834-01A1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10119365. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*